ICN: a normalization method for gene expression data considering the over-expression of informative genes

L Cheng, X Wang, PK Wong, KY Lee, L Li, B Xu… - Molecular …, 2016 - pubs.rsc.org
Normalization is a critical step in eliminating biases in microarray data for accurate analysis.
However, conventional normalization methods are usually not powerful in dealing with …

Selection of suitable reference genes for accurate normalization of gene expression profile studies in non-small cell lung cancer

S Saviozzi, F Cordero, ML Iacono, S Novello… - BMC cancer, 2006 - Springer
… the conclusion that for gene expression profiling in NSCLC the RGs most suitable for
normalization are rRNA18S, POLR2A, ESD and YAP1. For accurate normalization we recommend …

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

J Vandesompele, K De Preter, F Pattyn, B Poppe… - Genome biology, 2002 - Springer
… for high-throughput and accurate expression profiling of selected genes. Given the … gene
for normalization have become increasingly stringent. Although housekeeping gene expression

[HTML][HTML] Normalization of gene expression measurements in tumor tissues: comparison of 13 endogenous control genes

JB de Kok, RW Roelofs, BA Giesendorf… - Laboratory …, 2005 - Elsevier
genes. We recommend this gene for future studies to standardize gene expression
Our study clearly demonstrates that the expression of the HPRT gene accurately reflects the …

[PDF][PDF] Centralization: a new method for the normalization of gene expression data

A Zien, T Aigner, R Zimmer, T Lengauer - BIOINFORMATICS-OXFORD-, 2001 - Citeseer
… providing more diverse gene data, should allow for more accurate normalization. This effect
… most useful for the normalization of low gene number assays of gene expression, which can …

Evaluation of Reference Genes for Accurate Normalization of Gene Expression for Real Time-Quantitative PCR in Pyrus pyrifolia Using Different Tissue Samples and …

T Imai, BE Ubi, T Saito, T Moriguchi - PLoS One, 2014 - journals.plos.org
… We have shown in this study that gene expression stabilities are different among the sample
… for normalizing RT-qPCR data is needful. We propose that three best performing genes

Reliable gene expression analysis by reverse transcription-quantitative PCR: reporting and minimizing the uncertainty in data accuracy

T Remans, E Keunen, GJ Bex, K Smeets… - The Plant …, 2014 - academic.oup.com
… introduces a systematic error that affects the accuracy of the measurement. … data normalized
by reference gene expression is accurate only if all observed variation in the normalization

Validation of reference genes for accurate normalization of gene expression for real time-quantitative PCR in strawberry fruits using different cultivars and osmotic …

V Galli, JM Borowski, EC Perin, R da Silva Messias… - Gene, 2015 - Elsevier
genes for the normalization of gene expression in strawberry cultivars and under osmotic
stresses (salt and drought). Our results suggested that different suitable reference genesgenes

Comparison of normalization approaches for gene expression studies completed with high-throughput sequencing

F Abbas-Aghababazadeh, Q Li, BL Fridley - PloS one, 2018 - journals.plos.org
accuracy of statistical inferences from RNA-Seq data [16–18] and also prevent researchers
from properly modeling biological variation and group-specific changes in gene expression […

Accurate quantification of transcriptome from RNA-Seq data by effective length normalization

S Lee, CH Seo, B Lim, JO Yang, J Oh… - Nucleic acids …, 2011 - academic.oup.com
… As an indirect measure of prediction accuracy, we selected top 30 highly expressed genes
from … -known abundant genes such as GAPDH , ACTB and ribosomal genes. NEUMA’s top 30 …