[HTML][HTML] Identification and evaluation of new reference genes in Gossypium hirsutumfor accurate normalization of real-time quantitative RT-PCR data

S Artico, SM Nardeli, O Brilhante, MF Grossi-de-Sa… - BMC plant biology, 2010 - Springer
… spatial and temporal characterization of two MADS-box genes in cotton plants. In summary,
the new cotton reference genes will enable more accurate and reliable normalization of …

Evaluation and selection of reliable reference genes for gene expression under abiotic stress in cotton (Gossypium hirsutum L.)

M Wang, Q Wang, B Zhang - Gene, 2013 - Elsevier
… commonly used reference genes in upland cotton (Gossypium hirsutum) under salt and drought
… in Gossypium hirsutum for accurate normalization of real-time quantitative RT-PCR data …

[HTML][HTML] Genome wide search to identify reference genes candidates for gene expression analysis in Gossypium hirsutum

PK Smitha, K Vishnupriyan, AS Kar, M Anil Kumar… - BMC plant biology, 2019 - Springer
… PCR (qPCR) is the most reliable, accurate and cost-effective … An appropriate normalization
strategy like the selection of … very high leading to faulty normalization for low expressing target …

[HTML][HTML] Screening the Reference Genes for Quantitative Gene Expression by RT-qPCR During SE Initial Dedifferentiation in Four Gossypium hirsutum Cultivars that …

A Cao, D Shao, B Cui, X Tong, Y Zheng, J Sun, H Li - Genes, 2019 - mdpi.com
… If V n/n+1 = 0.15, the optimal number of reference genes for accurate normalization should
be optimal n+1; if V n/n+1 < 0.15, the optimal number of reference genes should be n [27]. As …

[HTML][HTML] Diversity analysis of cotton (Gossypium hirsutum L.) germplasm using the CottonSNP63K Array

LL Hinze, AM Hulse-Kemp, IW Wilson, QH Zhu… - BMC Plant …, 2017 - Springer
hirsutum from other Gossypium species and distinguished the wild from cultivated types of
G. hirsutum. … to providing the most accurate information to potential germplasm users. The ‘…

[HTML][HTML] Identifying Functional Genes Influencing Gossypium hirsutum Fiber Quality

C Dong, J Wang, Y Yu, L Ju, X Zhou, X Ma… - Frontiers in plant …, 2019 - frontiersin.org
… All SNP genotype data were treated with raw data normalization, clustering and genotype …
the accuracy of predicting complex quantitative traits. This work provided accurate phenotypic …

[HTML][HTML] Reference genome sequences of two cultivated allotetraploid cottons, Gossypium hirsutum and Gossypium barbadense

M Wang, L Tu, D Yuan, D Zhu, C Shen, J Li, F Liu… - Nature …, 2019 - nature.com
Allotetraploid cotton species (Gossypium hirsutum and Gossypium barbadense) have long
been cultivated worldwide for natural renewable textile fibers. The draft genome sequences …

Selection and validation of reliable reference genes in Gossypium raimondii

R Sun, Q He, B Zhang, Q Wang - Biotechnology letters, 2015 - Springer
… ), cDNA quality, primer specificity, and accurate normalization. Therefore, it is vital to …
Gossypium hirsutum and G. … genes as reference genes is not required for reliable normalization. …

A Long‐Read Transcriptome Assembly of Cotton (Gossypium hirsutum L.) and Intraspecific Single Nucleotide Polymorphism Discovery

H Ashrafi, AM Hulse‐Kemp, F Wang, SS Yang… - The plant …, 2015 - Wiley Online Library
… One microgram total RNA from TM-1 was used to prepare a partially normalized cDNA library
hirsutum genomes would be accurately represented by their putative diploid progenitors, …

Functional characterization of GhSOC1 and GhMADS42 homologs from upland cotton (Gossypium hirsutum L.)

X Zhang, S Fan, M Song, C Pang, H Wei, C Wang, S Yu - Plant Science, 2016 - Elsevier
… GhMADS42 (Gossypium hirsutum L.) were cloned from upland cotton CCRI36 and transformed
into Arabidopsis. GhSOC1 was additionally transformed into upland cotton. Comparative …