… The investigation of how random mutations may … a chromosomal background. Recently it
has been shown that PCR-generated mutations can be targeted to chromosomal Acinetobacter …

… In addition, 14 mutants were found to be auxotrophic (a frequency of 3 x 10'3). Thus, the … of
random mutants through insertion of a kanamycin resistance marker into the chromosome of A…

… The recombination events were non-randomly distributed in … antimicrobial resistance-conferring
mutations affecting the gyrA … and transposons or mutations in chromosomal genes. Our …

… library to screen mutants resistant to colistin is not a good choice, because A. baumannii
spontaneous chromosomal mutations are readily selected and result in resistant mutants after …

Background The emergence of multidrug resistant Acinetobacter baumannii within hospitals
poses a significant threat to patients. The inherent rate of mutation of these strains has not …

… contributed to divergence of this chromosomal gene (22). To calculate mutation frequency,
a direct … Mutants were constructed so that their pcaG genes contained variations in repeat …

… the mutated benM and catM chromosomal alleles of the parent strain (data not shown). …
Large spontaneously amplified chromosomal segments were detected in Acinetobacter at a …

… chromosome of the mutants To analyse the molecular structure of the chromosome of the
mutants, the npf//gene of each mutant, together with flanking chromosomal sequences, was …

… calcoaceticus is not well suited for transposon mutagenesis, a random mutagenesis … to
random fragments of chromosomal DNA. This method was used to generate several mutants …

… Whole genome phylogenetic analysis of 136 Acinetobacter genomes was used to shed light
… of pathogenesis associated genes in the Acinetobacter calcoaceticus-baumannii complex […