[HTML][HTML] Adhesion receptor expression by hematopoietic cell lines and murine progenitors: modulation by cytokines and cell cycle status
PS Becker, SK Nilsson, Z Li, VM Berrios… - Experimental …, 1999 - Elsevier
Hematopoietic progenitor cells are incubated with cytokine combinations for in vitro
expansion of stem cells and to enhance retrovirus-mediated gene transfer. Optimization of
the engraftment of these treated cells would be critical to the success of stem cell
transplantation or gene therapy. Previous studies demonstrated that a 48-hour incubation of
donor BALB/c bone marrow with a mixture of four cytokines (IL-3, IL-6, IL-11, and SCF),
resulted in expansion of primitive progenitor/stem cells but a loss of long-term engraftment in …
expansion of stem cells and to enhance retrovirus-mediated gene transfer. Optimization of
the engraftment of these treated cells would be critical to the success of stem cell
transplantation or gene therapy. Previous studies demonstrated that a 48-hour incubation of
donor BALB/c bone marrow with a mixture of four cytokines (IL-3, IL-6, IL-11, and SCF),
resulted in expansion of primitive progenitor/stem cells but a loss of long-term engraftment in …
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