Increasing mixed chimerism (MC) after allogeneic stem cell transplantation (SCT) has been
associated with a high risk of relapse in acute leukemia. We evaluated a new method for …

… We conclude that the qPCR chimerism assay for engraftment monitoring is a reliable and
sensitive assay. Advantages and limitations of the assay in its current format are summarized. …

… donor act as a specific tag to identify and quantify the presence of specific cell types in the
… , and more reliable results are obtained for minor genotype detection and quantification than …

… Duplex PCRs were carried out to facilitate reliable quantification … Standard curves for
quantification were generated by mixing … MRD quantification was carried out using Excel software (…

… cells reliably detected by the system was 0.01% for both qPCR and dPCR. The limit …
quantification (LOQ), defined as the lowest amount of host blood cells that can be reliably quantified (…

… after allogeneic stem cell transplantation to … reliable accuracy, but is hampered by its limited
sensitivity (≥1%). Digital PCR (dPCR) has been shown to combine exact quantification and …

… fluorescent primers enables reliable quantification without the need for additional densitometry.
The method can be especially helpful in monitoring patients undergoing transplantation. …

… The development of a fluorometric method for cycleto-cycle quantification of PCR product …
reliable quantification of nucleic acid sequences. In contrast to current end-point quantification …

… This duplex PCR assay allows the quantification of TREC and KREC in the same PCR …
to test reliable TREC/KREC values. Especially during neutropenia cell numbers were limiting. …

… ) BCR-ABL quantification to predict relapse of CML patients receiving allogeneic SCT. Minimal
… an RQ-PCR amplifiability index and the limits of reliable use of BCR-ABL ratios. We then …