High efficient multisites genome editing in allotetraploid cotton (Gossypium hirsutum) using CRISPR/Cas9 system

P Wang, J Zhang, L Sun, Y Ma, J Xu… - Plant biotechnology …, 2018 - Wiley Online Library
… /Cas9 system is highly efficient and reliable for allotetraploid cotton genome editing. …
CRISPR/Cas9 system in allotetraploid cotton and accomplished multiple sites genome editing. The …

[HTML][HTML] Robust CRISPR/Cpf1 (Cas12a)‐mediated genome editing in allotetraploid cotton (Gossypium hirsutum)

B Li, H Rui, Y Li, Q Wang, M Alariqi, L Qin… - Plant biotechnology …, 2019 - ncbi.nlm.nih.gov
cotton genome editing. Overall, in this study, we successfully built a site genome editing in
allotetraploid cotton with 87% editing … Our results are comparable with the editing efficiency in …

High‐efficient and precise base editing of C•G to T•A in the allotetraploid cotton (Gossypium hirsutum) genome using a modified CRISPR/Cas9 system

L Qin, J Li, Q Wang, Z Xu, L Sun… - Plant biotechnology …, 2020 - Wiley Online Library
… Comparing the off-target effects in previous reports, the GhBE3 system used in this report
is highly precise for cotton genome editing. The negligible C→T conversions at these off-target …

[HTML][HTML] Development of an efficient and precise adenine base editor (ABE) with expanded target range in allotetraploid cotton (Gossypium hirsutum)

G Wang, Z Xu, F Wang, Y Huang, Y Xin, S Liang, B Li… - BMC biology, 2022 - Springer
… To investigate the genome editing specificity of ABEs in cotton, two edited plants
generated by the GhABE7.10n and GhABE7.10d vectors were chose to determine the off-target …

[HTML][HTML] Genome editing in cotton: challenges and opportunities

Z Khan, SH Khan, A Ahmed, MU Iqbal… - Journal of Cotton …, 2023 - Springer
… In this review, a general description of the available genome editing platforms has been
given with special emphasis on using these platforms for genetic improvement in cotton. …

[HTML][HTML] Genome editing in cotton with the CRISPR/Cas9 system

W Gao, L Long, X Tian, F Xu, J Liu, PK Singh… - Frontiers in plant …, 2017 - frontiersin.org
cotton genes, an important requisite in this allotetraploid crop. We also show that multiple
gene targeting can be achieved in cotton … to produce targeted gene fragment deletions in the …

Targeted genome editing for cotton improvement: prospects and challenges

R Kumar, J Das, RK Puttaswamy, M Kumar… - The Nucleus, 2024 - Springer
… facilitated the development of high-oleic, non-transgenic mutants in allotetraploid upland
cotton with an editing efficiency as high as 54% [29]. These transgene-free mutants hold …

[HTML][HTML] … sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypol‐free cotton

B Li, S Liang, M Alariqi, F Wang, G Wang… - Plant Biotechnology …, 2021 - ncbi.nlm.nih.gov
… However, the temperature sensitivity of LbCpf1 has not been tested in cotton yet, a high
temperature‐resistant crop. In order to improve LbCpf1 efficiency and determine the …

Genome editing in cotton using CRISPR/Cas9 system

C Li, B Zhang - Transgenic Cotton: Methods and Protocols, 2019 - Springer
… Recently, CRISPR/Cas9-mediated genome editing system … targeted gene mutation in
allotetraploid cotton genome using … -like D that derived from upland cotton (Gossypium hirsutum) A …

[HTML][HTML] Highly efficient genome editing using geminivirus-based CRISPR/Cas9 system in cotton plant

B Li, C Fu, J Zhou, F Hui, Q Wang, F Wang, G Wang… - Cells, 2022 - mdpi.com
… More than 90% of fiber products come from allotetraploid cotton (G. hirsutum and G.
barbadense), which originated from an allopolyplodization event approximately 1–2 million year ago…