High efficient multisites genome editing in allotetraploid cotton (Gossypium hirsutum) using CRISPR/Cas9 system
P Wang, J Zhang, L Sun, Y Ma, J Xu… - Plant biotechnology …, 2018 - Wiley Online Library
… /Cas9 system is highly efficient and reliable for allotetraploid cotton genome editing. …
CRISPR/Cas9 system in allotetraploid cotton and accomplished multiple sites genome editing. The …
CRISPR/Cas9 system in allotetraploid cotton and accomplished multiple sites genome editing. The …
[HTML][HTML] Robust CRISPR/Cpf1 (Cas12a)‐mediated genome editing in allotetraploid cotton (Gossypium hirsutum)
B Li, H Rui, Y Li, Q Wang, M Alariqi, L Qin… - Plant biotechnology …, 2019 - ncbi.nlm.nih.gov
… cotton genome editing. Overall, in this study, we successfully built a site genome editing in
allotetraploid cotton with 87% editing … Our results are comparable with the editing efficiency in …
allotetraploid cotton with 87% editing … Our results are comparable with the editing efficiency in …
High‐efficient and precise base editing of C•G to T•A in the allotetraploid cotton (Gossypium hirsutum) genome using a modified CRISPR/Cas9 system
… Comparing the off-target effects in previous reports, the GhBE3 system used in this report
is highly precise for cotton genome editing. The negligible C→T conversions at these off-target …
is highly precise for cotton genome editing. The negligible C→T conversions at these off-target …
[HTML][HTML] Development of an efficient and precise adenine base editor (ABE) with expanded target range in allotetraploid cotton (Gossypium hirsutum)
… To investigate the genome editing specificity of ABEs in cotton, two edited plants
generated by the GhABE7.10n and GhABE7.10d vectors were chose to determine the off-target …
generated by the GhABE7.10n and GhABE7.10d vectors were chose to determine the off-target …
[HTML][HTML] Genome editing in cotton: challenges and opportunities
… In this review, a general description of the available genome editing platforms has been
given with special emphasis on using these platforms for genetic improvement in cotton. …
given with special emphasis on using these platforms for genetic improvement in cotton. …
[HTML][HTML] Genome editing in cotton with the CRISPR/Cas9 system
… cotton genes, an important requisite in this allotetraploid crop. We also show that multiple
gene targeting can be achieved in cotton … to produce targeted gene fragment deletions in the …
gene targeting can be achieved in cotton … to produce targeted gene fragment deletions in the …
Targeted genome editing for cotton improvement: prospects and challenges
… facilitated the development of high-oleic, non-transgenic mutants in allotetraploid upland
cotton with an editing efficiency as high as 54% [29]. These transgene-free mutants hold …
cotton with an editing efficiency as high as 54% [29]. These transgene-free mutants hold …
[HTML][HTML] … sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypol‐free cotton
… However, the temperature sensitivity of LbCpf1 has not been tested in cotton yet, a high
temperature‐resistant crop. In order to improve LbCpf1 efficiency and determine the …
temperature‐resistant crop. In order to improve LbCpf1 efficiency and determine the …
Genome editing in cotton using CRISPR/Cas9 system
… Recently, CRISPR/Cas9-mediated genome editing system … targeted gene mutation in
allotetraploid cotton genome using … -like D that derived from upland cotton (Gossypium hirsutum) A …
allotetraploid cotton genome using … -like D that derived from upland cotton (Gossypium hirsutum) A …
[HTML][HTML] Highly efficient genome editing using geminivirus-based CRISPR/Cas9 system in cotton plant
B Li, C Fu, J Zhou, F Hui, Q Wang, F Wang, G Wang… - Cells, 2022 - mdpi.com
… More than 90% of fiber products come from allotetraploid cotton (G. hirsutum and G.
barbadense), which originated from an allopolyplodization event approximately 1–2 million year ago…
barbadense), which originated from an allopolyplodization event approximately 1–2 million year ago…
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