Accuracy of an amplicon-sequencing nanopore approach to identify variants in tuberculosis drug-resistance-associated genes

C Mariner-Llicer, GA Goig… - Microbial …, 2021 - microbiologyresearch.org
drug resistance and genotype at almost real-time. This work describes a proof-of-concept
study in which a single-tube PCR assay to identify drug resistance … long-amplicon sequencing

Rapid drug susceptibility testing of drug-resistant Mycobacterium tuberculosis isolates directly from clinical samples by use of amplicon sequencing: a proof-of-concept …

RE Colman, J Anderson, D Lemmer… - Journal of clinical …, 2016 - Am Soc Microbiol
… the advancement of amplicon sequencing using overlapping … drug-resistant bacteria (20).
This pilot study evaluates the performance and clinical utility of rapid amplicon sequencing of …

A method for amplicon deep sequencing of drug resistance genes in Plasmodium falciparum clinical isolates from India

PN Rao, S Uplekar, S Kayal, PK Mallick… - Journal of clinical …, 2016 - Am Soc Microbiol
… Next-generation sequencing (NGS) methods provide the resolution, scalability, and …
molecular markers of drug resistance. We have developed an amplicon sequencing method on the …

Amplicon Sequencing as a Potential Surveillance Tool for Complexity of Infection and Drug Resistance Markers in Plasmodium falciparum Asymptomatic Infections

K Wamae, KM Kimenyi, V Osoti… - The Journal of …, 2022 - academic.oup.com
… first febrile infections using amplicon sequencing (AmpSeq) of ama1 in Coastal Kenya. We
also examined temporal changes in haplotype frequencies of mdr1, a drug-resistant marker. …

[HTML][HTML] Detection of Low-Level Mixed-Population Drug Resistance in Mycobacterium tuberculosis Using High Fidelity Amplicon Sequencing

RE Colman, JM Schupp, ND Hicks, DE Smith… - PloS one, 2015 - journals.plos.org
… provides increased sensitivity over standard DNA sequencing. Ligation free amplicon
sequencing with SMOR analysis enables the detection of resistant allele subpopulations at ≥0.1…

[HTML][HTML] Next-generation sequencing and PCR technologies in monitoring the hospital microbiome and its drug resistance

C Cason, M D'Accolti, I Soffritti, S Mazzacane… - Frontiers in …, 2022 - frontiersin.org
sequencing applications can be applied to hospital surveillance and can include amplicon
sequencing… currently in use are amplicon sequencing and shotgun metagenomic …

[HTML][HTML] Molecular surveillance of Plasmodium falciparum drug-resistance markers in Vietnam using multiplex amplicon sequencing (2000–2016)

E Rovira-Vallbona, JH Kattenberg, NV Hong… - Scientific Reports, 2023 - nature.com
… Controlling the expansion of drug resistant lineages is critical to sustain gains in malaria …
multiplex amplicon sequencing approach for high-throughput screening of resistance markers …

[HTML][HTML] Clinical utility of target amplicon sequencing test for rapid diagnosis of drug-resistant Mycobacterium tuberculosis from respiratory specimens

KSS Leung, KKG Tam, TTL Ng, HY Lao… - Frontiers in …, 2022 - frontiersin.org
… Although the discrepancies could be resolved by Sanger sequencing, the process is usually
… a target amplicon sequencing protocol (TB-NGS) specifically targeting drug resistance-…

Deep amplicon sequencing for culture-free prediction of susceptibility or resistance to 13 anti-tuberculous drugs

A Jouet, C Gaudin, N Badalato… - European …, 2021 - Eur Respiratory Soc
… Phenotypic drug susceptibility testing (pDST) takes weeks … drug resistance mutations in a
few gene targets [2]. Whole-genome sequencing (WGS) can effectively predict drug resistance

Cost-efficient HIV-1 drug resistance surveillance using multiplexed high-throughput amplicon sequencing: implications for use in low-and middle-income countries

H Ekici, SD Rao, A Sönnerborg… - Journal of …, 2014 - academic.oup.com
Objectives Increased trends of primary drug resistance mutations (DRMs) among treatment-naive
HIV-1-infected patients in low- and middle-income countries (LMICs) and the non-…