… we used confocal fluorescence microscopy and SIM to … super-resolution fluorescence
imaging, however, we have been able to define important details of the intracellular aggregates …

… stochastic optical reconstruction microscopy, dSTORM, … microscopy techniques. It is reviewed
later in this chapter in a section headed Super-resolution imaging of amyloid aggregation; …

… Conventional fluorescence microscopy is limited in resolution by the diffraction of light; in
an optical system with finite aperture, this resolution limit is typically in the 250 nm range. An …

… In Figure 4c, we report the distribution of the measured lengths of individual Aβ 40 amyloid
aggregates at sampling points t 1 –t 4 along the assembly pathway, detected from the cluster …

… to an imaging chamber by using an epi-fluorescence microscope with a highly inclined 488-nm
… stages of the amyloid aggregation process. We generated Aβ40 aggregates from the late …

… of the aggregation of monomeric β-amyloid … super-resolution images (right). The
intensity profiles of the dash lines in the conventional fluorescence microscopic and super-resolution …

… detection and imaging in vivo of amyloid aggregates, which are involved in … Super-resolution
fluorescence microscopy techniques such as STimulated Emission Depletion microscopy …

… agreement with antibodies specific for amyloid-β or α-… fluorescence microscopy with
fluorophores such as Thioflavin S and Thioflavin T (ThT) has enabled detection of aggregate …

… changing state of amyloid aggregation in live samples. Next, … the ability of super-resolution
microscopy at detecting and … fluorescence microscopy in the studies of protein aggregates in …

… fluorescent marker to detect aggregation of amyloid fibrils by conventional fluorescence
microscopy… of the ThT fluorescence emission under adequate buffer conditions permit stochastic …