Fluorescence lifetime measurements and biological imaging

MY Berezin, S Achilefu - Chemical reviews, 2010 - ACS Publications
… This section briefly describes the basic method of fluorescence lifetime measurements. … ,
particularly, autofluorescence lifetime imaging. Autofluorescence lifetime imaging is an attractive …

Molecular imaging in living subjects: seeing fundamental biological processes in a new light

TF Massoud, SS Gambhir - Genes & development, 2003 - genesdev.cshlp.org
… A further incentive for use of molecular imaging strategies in basic biological research is the
… to autofluorescence of tissues in fluorescence imaging as compared with bioluminescence …

Quantitative NAD (P) H/flavoprotein autofluorescence imaging reveals metabolic mechanisms of pancreatic islet pyruvate response

JV Rocheleau, WS Head, DW Piston - Journal of Biological Chemistry, 2004 - ASBMB
… Using this multicolor autofluorescence imaging method, we demonstrate that glucose
stimulation results in concomitant production of NADH and NADPH. In contrast, pyruvate …

Virtual histological staining of unlabelled tissue-autofluorescence images via deep learning

Y Rivenson, H Wang, Z Wei, K de Haan… - Nature biomedical …, 2019 - nature.com
autofluorescence images of label-free tissue sections that did not overlap with the images
… the virtual-staining method on the basis of a contrast mechanism that originates from tissue …

[HTML][HTML] Spatial and spectral characterization of human retinal pigment epithelium fluorophore families by ex vivo hyperspectral autofluorescence imaging

TB Ami, Y Tong, A Bhuiyan, C Huisingh… - … vision science & …, 2016 - iovs.arvojournals.org
… Translational Relevance: Our results help establish hyperspectral autofluorescence imaging
of the human retinal pigment epithelium as a useful tool for investigating retinal health and …

Fluorescence and spectral imaging

RS DaCosta, BC Wilson… - The scientific world journal, 2007 - Wiley Online Library
… In Barrett’s esophagus, the detection of dysplasia remains problematic because of background
inflammation, whereas in the squamous esophagus, autofluorescence imaging appears …

Autofluorescence microscopy of fresh cervical‐tissue sections reveals alterations in tissue biochemistry with dysplasia¶

R Drezek, C Brookner, I Pavlova, I Boiko… - Photochemistry and …, 2001 - Wiley Online Library
Autofluorescence images were acquired at 380 … biological basis of the clinically measured
spectra. In this paper we expand upon our previous work by considering the autofluorescence

Autofluorescence patterns in short‐term cultures of normal cervical tissue

CK Brookner, M Follen, I Boiko, J Galvan… - Photochemistry and …, 2000 - Wiley Online Library
… to characterize normal cervical autofluorescence, using fresh … 31 cervical biopsies;
autofluorescence images were obtained at … These results suggest a biological basis for the …

Trends in fluorescence imaging and related techniques to unravel biological information

E Haustein, P Schwille - HFSP journal, 2007 - Taylor & Francis
… It is indispensable for basic lab work, as well as for cutting edge … imaging whole fruit flies
and mouse embryos using only autofluorescence, but they could also reconstruct 3D images of …

Device and programs for investigation of biological objects autofluorescence

AA Klimov, DA Klimov - Biophysics, 2012 - Springer
… mole cules superimposes on the image created by intrinsic fluorescence of the object. This
autofluorescence is noise, not permitting «cleansing» the image of spot we seek for to such a …