Development of real-time RT-PCR for the detection of avian influenza virus

E Spackman, DA Senne, LL Bulaga… - Avian …, 2003 - meridian.allenpress.com
… North American avian influenza viruses. The sensitivity and specificity of this RRT-PCR assay
… RRT-PCR detected influenza in samples from 61 of 65 (93.8%) of the LBMs that were the …

Type‐and subtype‐specific RTPCR assays for avian influenza A viruses (AIV)

E Starick, A Rømer‐Oberdørfer… - Journal of Veterinary …, 2000 - Wiley Online Library
… The RT-PCR technique was also used prior to sequencing of … -specific RT-PCR assays
for H5 and H7 avian influenza … -specific RT-PCR has been established to improve influenza

The effect of various disinfectants on detection of avian influenza virus by real time RT-PCR

DL Suarez, E Spackman, DA Senne… - Avian …, 2003 - meridian.allenpress.com
… -polymerase chain reaction (RRT-PCR) test was previously … The test can also be used to
identify avian influenza virus (… RRT-PCR was being considered as a component of the influenza

Real-time RT-PCR for H5N1 avian influenza A virus detection

W Chen, B He, C Li, X Zhang, W Wu… - Journal of medical …, 2007 - microbiologyresearch.org
… pathogenic avian influenza virus A H5N1real-time RT-PCR method for the detection of
influenza A subtype H5 and for monitoring virus loads. Using serial dilutions of influenza A H5N1

Development of a real-time TaqMan RT-PCR assay for the detection of H9N2 avian influenza viruses

MB Shabat, R Meir, R Haddas, E Lapin… - Journal of Virological …, 2010 - Elsevier
Avian influenza viruses (AIVs) of the H9N2 subtype are a major … This study aimed to develop
a real-time TaqMan ® RT-PCRavian pathogens, and was found specific to H9N2. The …

Development of multiplex real-time RT-PCR as a diagnostic tool for avian influenza

E Spackman, DA Senne, LL Bulaga, S Trock… - Avian …, 2003 - meridian.allenpress.com
… A multiplex real-time reverse transcriptase-polymerase chain reaction (RRT-PCR) assay
for the simultaneous detection of the H5 and H7 avian influenza hemagglutinin (HA) subtypes …

Avian influenza virus detection and quantitation by real-time RT-PCR

E Spackman - Animal influenza virus, 2014 - Springer
polymerase chain reaction is the same for real-time PCR as it is for conventional PCR. The
difference between the two methods is in how the PCR product (amplicon) is detected. With …

Avian influenza virus detection and quantitation by real-time RT-PCR

E Spackman - Animal Influenza Virus: Methods and Protocols, 2020 - Springer
Real-time reverse transcription PCR (rRT-PCR) was initially introduced for avian influenza
an outbreak of low-pathogenic (LP) avian influenza virus (AIV) in commercial poultry in 2002 […

Identification and subtyping of avian influenza viruses by reverse transcription-PCR

MS Lee, PC Chang, JH Shien, MC Cheng… - Journal of virological …, 2001 - Elsevier
… of avian influenza virus by reverse transcription-PCR (RT-PCR). The avian influenza virus is
identified by RT-PCR … nucleoprotein (NP) gene of avian influenza virus. The HA-subtypes of …

Development of a real-time RT-PCR method for rapid detection of H9 avian influenza virus in the air

J Lv, B Wei, T Chai, X Xia, Z Miao, M Yao, Y Gao… - Archives of …, 2011 - Springer
real-time RT-PCR, and their mean concentrations ranged from 1.25×10 4 to 6.92×10 4 copies/m
3 air. Overall, the real-time PCR … [10] discovered the airborne avian influenza virus type …