… ), such that DNA can be introduced to the bacterial cell. By contrast, in this … transformation,
in which specialized bacterial proteins are responsible for the uptake and processing of DNA…

… However, there is no evidencethat these proteins are involved in transformation, and, because
DNA uptake inthese organisms requires a specific sequence, a ComEA ortholog may not …

… the bacterial cell ( Figures 1 and 2 ). We use the term uptake to mean entry of transforming
DNA … Bacterial transformation: ComFA is a DNA-dependent ATPase that forms complexes with …

… The emphasis of this review will be on DNA uptake by the natural mechanisms of bacterial
transformation. Research … Natural bacterial transformation Genetic transformation by purified …

… DNA uptake has been characterized to various extents in both Gram-positive, … DNA uptake
complex comes from studies in B. subtilis (Dubnau, 1999). In a working model for DNA uptake …

… Gram-positive bacteria are able to acquire plasmid DNA under the Yoshida effect. Therefore,
it is possible that this protocol may be widely applicable for bacterial transformations with …

… initial process of DNA uptake, regardless of the fate imported DNA faces in the interior of the
… Here we propose that DNA uptake proceeds in two steps in all transformable bacteria and …

… the transformation process and subsequently go into detail about what is known about DNA
uptake … the relation between natural transformation and type-IV pili can not yet be addressed. …

… use for DNA uptake and processing, but natural competence and transformation have never
… homologues encode a functional DNA uptake machinery and whether DNA uptake leads to …

… DNA uptake, we first inactivated the hofQ gene as described in Materials and Methods.
Comparison of the plasmid transformation … of conserved bacterial transformation machineries (Fig…