… base editor shortcomings, including off-target DNA editing, … , which arise from the main DNA
base editor constituents, a Cas … The activity of ABE7.10 was further enhanced by addition of …

… base is located within the base editor activity window relative to the protospacer adjacent
motif (PAM) site; there is only one target base in the activity window, and the target base … base …

… b | Activity windows of base editors with the basic architecture from part a with the indicated
Cas … Base editor activity windows are shown over the 20-nucleotide protospacer sequence (…

… activity in living cells. We show that GO can be used to directly and quantitatively compare the
efficiency, off-target activity… streamline the application of base editing activity in primary and …

… Base editors (BEs) consist of cytosine deaminases and uracil … activities of enAsCas12a
could enable efficient base editing, we compared four different denAsCas12a base-editor fusions …

… Although base editors are widely used to install … base editing outcomes are not well
understood. We characterized sequence-activity relationships of 11 cytosine and adenine base …

… We next investigated whether we could discriminate the editing activity windows and editing
patterns induced by ABE/CBE RNPs and by plasmid-encoded ABE/CBEs. An analysis of the …

… base editor, AYBE, for A-to-C and A-to-T transversion editing in mammalian cells by fusing
an adenine base editor (ABE) … editing activity and product purity of AYBE to obtain an A-to-C …

… we examined the editing activities by catalytic inactivation of only the wild-type monomer (via
an F148A mutation), and found that this variant maintained DNA on-target activity but could …

… editing efficiency, limited editing scopes, and off-targeting effects. Here, we develop a new
adenine base editor… -8e-SpRY, which exhibits higher activity at NRN than NYN PAMs favored …