[PDF][PDF] Precise in vivo RNA base editing with a wobble-enhanced circular CLUSTER guide RNA
P Reautschnig, C Fruhner, N Wahn, CP Wiegand… - Nature …, 2024 - nature.com
… editing precision and efficiency. We apply G•U wobble base pairs in various RNA base-editing
approaches including the CLUSTER approach, where it greatly improves the guide RNA …
approaches including the CLUSTER approach, where it greatly improves the guide RNA …
Improving the precision of base editing by bubble hairpin single guide RNA
Z Hu, Y Wang, Q Liu, Y Qiu, Z Zhong, K Li, W Li, Z Deng… - MBio, 2021 - Am Soc Microbiol
… Furthermore, our sgRNA design also dramatically reduced indels and unwanted base …
sgRNAs of base editors, and our design promises to be adaptable to various base editors. We …
sgRNAs of base editors, and our design promises to be adaptable to various base editors. We …
Precision RNA base editing with engineered and endogenous effectors
LS Pfeiffer, T Stafforst - Nature Biotechnology, 2023 - nature.com
… effectors are usually composed of the DD of an ADAR enzyme, a target-specific guide RNA
(gRNA) and a protein module fused to the DD that tethers the effector protein to the gRNA. …
(gRNA) and a protein module fused to the DD that tethers the effector protein to the gRNA. …
Chemical modifications of adenine base editor mRNA and guide RNA expand its application scope
T Jiang, JM Henderson, K Coote, Y Cheng… - Nature …, 2020 - nature.com
… base editor that mediates robust editing at various cellular genomic sites together with
moderately modified guide RNA, and … of adenine base editor mRNA and guide RNA expand the …
moderately modified guide RNA, and … of adenine base editor mRNA and guide RNA expand the …
PnB Designer: a web application to design prime and base editor guide RNAs for animals and plants
… in other base editing … base editor options to choose new PAMs and editing windows, we
think implementation of the newest base editors with their experimentally established editing …
think implementation of the newest base editors with their experimentally established editing …
beditor: A Computational Workflow for Designing Libraries of Guide RNAs for CRISPR-Mediated Base Editing
… of the software by designing guide RNAs for base editing to model or … base editors and
PAM recognition sequences. To demonstrate the utility of beditor in utilizing custom base editors …
PAM recognition sequences. To demonstrate the utility of beditor in utilizing custom base editors …
Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors
… in editing efficiency. Finally, we show that an adenine base editor 7 can also induce
transcriptome-wide RNA … Guide RNA oligonucleotides used in this study are listed in …
transcriptome-wide RNA … Guide RNA oligonucleotides used in this study are listed in …
Base editing: precision chemistry on the genome and transcriptome of living cells
… The continued development of context-dependent base editors 101 or future base editor
variants that lack the ability to bind ssDNA without assistance from the guide RNA represents a …
variants that lack the ability to bind ssDNA without assistance from the guide RNA represents a …
Off-target editing by CRISPR-guided DNA base editors
SH Park, PA Beal - Biochemistry, 2019 - ACS Publications
… DNA base editors. In this Perspective, we discuss the development of DNA base editors, the
guide RNA-… in recent studies, and strategies that improve the selectivity of DNA base editors. …
guide RNA-… in recent studies, and strategies that improve the selectivity of DNA base editors. …
Guidelines for C to T base editing in plants: base-editing window, guide RNA length, and efficient promoter
BC Kang, JW Woo, ST Kim, SJ Bae, M Choi… - Plant Biotechnology …, 2019 - Springer
… system was much efficient than 35S promoter-driven base-editor system. This study provides
guidelines on which a base editor to use and describes how to fine-tune a guide RNA for …
guidelines on which a base editor to use and describes how to fine-tune a guide RNA for …
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