Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions
… Base editing induces single-nucleotide changes in the DNA of living cells using a fusion
protein containing a catalytically defective Streptococcus pyogenes Cas9, a cytidine deaminase…
protein containing a catalytically defective Streptococcus pyogenes Cas9, a cytidine deaminase…
DNA base editing in nuclear and organellar genomes
… editing reagents based on transcription activator-like effectors has enabled the extension of
base editing to the … In this review, we summarize currently available base editing methods for …
base editing to the … In this review, we summarize currently available base editing methods for …
Controllable genome editing with split-engineered base editors
KN Berríos, NH Evitt, RA DeWeerd, D Ren… - Nature chemical …, 2021 - nature.com
… of targeted genome editing in living cells. Temporal control over base editing would allow for
… To evaluate if the seBE complex could be used to lie dormant in a cell line until base editing …
… To evaluate if the seBE complex could be used to lie dormant in a cell line until base editing …
Base editing mediated generation of point mutations into human pluripotent stem cells for modeling disease
T Qi, F Wu, Y Xie, S Gao, M Li, J Pu, D Li… - Frontiers in cell and …, 2020 - frontiersin.org
… Base editing is a … of living cells. Here, we design an all-in-one episomal vector that expresses
a single guide RNA (sgRNA) with an adenine base editor (ABE) or a cytosine base editor (…
a single guide RNA (sgRNA) with an adenine base editor (ABE) or a cytosine base editor (…
Base editing: Chemistry on a target nucleotide in the genome of living cells
D Liu - The FASEB Journal, 2019 - Wiley Online Library
… cells. We also show that base editing can function in vivo in post-mitotic somatic cells that do
not … To improve the targeting scope of base editing, we used our phage-assisted continuous …
not … To improve the targeting scope of base editing, we used our phage-assisted continuous …
Base editing the mammalian genome
… DNA base editing, and discuss the recent progress toward the development of optimized base
editing systems for mammalian cells. … examples of successful base editing in cell lines and …
editing systems for mammalian cells. … examples of successful base editing in cell lines and …
Base editing and prime editing in laboratory animals
F Caso, B Davies - Laboratory animals, 2022 - journals.sagepub.com
… editing in animal research studies. Both cytosine and adenine base editors have been applied
successfully to the editing of … Similarly, both base editors have achieved precise editing of …
successfully to the editing of … Similarly, both base editors have achieved precise editing of …
Current status and challenges of DNA base editing tools
… base editor constituents, a Cas nuclease variant and a deaminase. In this review, we summarize
information about the DNA base editors … or mitigate those issues of DNA base editors. …
information about the DNA base editors … or mitigate those issues of DNA base editors. …
A split and inducible adenine base editor for precise in vivo base editing
… DNA base editors use deaminases fused to a programmable DNA-binding protein for …
post-translational control in living cells. Here, we present a split adenine base editor (sABE) that …
post-translational control in living cells. Here, we present a split adenine base editor (sABE) that …
Therapeutic adenine base editing of human hematopoietic stem cells
J Liao, S Chen, S Hsiao, Y Jiang, Y Yang… - Nature …, 2023 - nature.com
… We observed highly efficient on-target adenine base edits at these two regulatory regions,
resulting in robust γ-globin induction. Moreover, we developed ABE8e-SpRY, a near-PAMless …
resulting in robust γ-globin induction. Moreover, we developed ABE8e-SpRY, a near-PAMless …