… of any living cell with minimal unwanted genome modification or cellular perturbation. Our
objectives are to present readers with an overview of the capabilities and limitations of current …

… Here, we test the ability of the Target-AID base editor to systematically modify genes genome…
gRNAs in the context of base editing. Our results show that base editing is a powerful tool to …

… Two groups of base editors exist DNA and RNA base editors. … responsible for modifying the
base. Two DNA base editors are currently in use: the cytidine base editor (CBE) and adenine …

… known as base editing, opened new avenues for microbial genome engineering. Base editing
… Here, we review ongoing efforts to develop and apply base editing to engineer industrially …

… cell therapy with potentially improved functionality, 12 we further demonstrated that base
editing … Tumor cell fluorescence was measured using the Incucyte live cell imaging system as a …

… -231 cells that stably expressed one of nine base editors that span a range of PAM specificities,
editing window sizes and overall editing … We next transduced each base editor- or Cas9-…

… To implement GSE in mammalian cells, we developed a multi-site base editing protocol.
We introduced targeted edits using adenine base editors (ABEs) and cytosine base editors (…

… Briefly, we gated on the live cell population (identified based on their characteristic
forward/side scatter pattern) and then displayed its GFP vs BFP expression as a bivariate contour …

… Engraftment was defined as >0.5 to 1% circulating huCD45 + cells of live cells. Both
patient AML samples were phenotypically identified as CD33 + /CD45 dim (compared with …

… Furthermore, our data suggested that the indels of base editing are … the base editing
mechanism, this work first elucidates the key roles of TLS polymerases in the cytosine base editing …