… 50% threshold) and the nucleotide distribution across all reads. In our pipeline, we used
FastQC (with default Phred = 20 value) as it plots the read depth and quality score besides a …

… Capture Processor (SECAPR) pipeline for processing raw sequencing data into multiple
sequence … SECAPR is an easy-to-use, free, and versatile pipeline, aimed to enable efficient and …

… To resolve this problem, we offer a program, TreeToReads, which can generate raw read
data from mutated genomes simulated under a known phylogeny. This simulation pipeline …

… read counts internally, while read counts for STAR and Tophat2/Bowtie2 were generated
using htseqcount. Computed gene read counts were compared to the exact gene read … the raw …

… The GS FLX+ run produced the lowest number of raw reads (1/8 of a plate) compared to
PGM (314X chip) and MiSeq (one lane); however, quality scores were higher in comparison to …

… (s) with the raw NGS reads. … pipelines, thus allowing the use of any software package(s) to
simulate the original sequence data and the NGS short reads, or to perform the bioinformatics …

… a complex bioinformatics analysis, the raw data and the list … regions and to merge overlapping
reads. We have also … ], to discriminate between human reads and mouse host reads in the …

… pipelines and there is a lack of agreement regarding best practices. Here, we compared six
bioinformatic pipelines … Raw reads were imported into a Qiime2 artifact before merging paired…

… studies rely on the raw data as it delivers a prompt insight of the sequence quality for further
downstream analysis. In our pipeline, we used FastQC as it plots the read depth and quality …

… For each nucleotide sequenced in these short fragments (ie, raw reads), a corresponding
Phred-like quality score is assigned, which is sequencing platform specific. The read …