Time-domain fluorescence lifetime imaging applied to biological tissue

D Elson, J Requejo-Isidro, I Munro, F Reavell… - Photochemical & …, 2004 - Springer
… in fluorescence intensity across the image, we conclude that the observed variation in
fluorescence lifetime … 3(b) is not likely to be significant but the lifetime difference observed at the ‘…

Non-linear fluorescence lifetime imaging of biological tissues

R Cicchi, F Saverio Pavone - Analytical and bioanalytical chemistry, 2011 - Springer
… still to be done, including the interpretation of fluorescence lifetime data, we believe that this
… by using fluorescence lifetime imaging microscopy for the investigation of biological tissues. …

Fluorescence lifetime measurements and biological imaging

MY Berezin, S Achilefu - Chemical reviews, 2010 - ACS Publications
… than elastin, as illustrated in Figure 29, where the mucosal surface composed of elastin
shows an excellent contrast in fluorescence lifetime with collagen rich tissue. In addition to …

Studying biological tissue with fluorescence lifetime imaging: microscopy, endoscopy, and complex decay profiles

J Siegel, DS Elson, SED Webb, KCB Lee, A Vlandas… - Applied …, 2003 - opg.optica.org
… study of biological tissue and … fluorescence lifetime data from real tissues, we present in
vitro microscopy studies of the complex fluorescence decay profiles observed in biological tissue

Multi‐wavelength fluorescence lifetime spectroscopy: a new approach to the study of endogenous fluorescence in living cells and tissues

D Chorvat Jr, A Chorvatova - Laser Physics Letters, 2009 - Wiley Online Library
… can serve to demarcate specific cell types in tissues, or organs, as well as their changes
in … between fluorescence lifetimes or spectra of endogenous fluorophores and biological

Portable fluorescence lifetime spectroscopy system for in-situ interrogation of biological tissues

M Saito Nogueira, A Cosci… - Journal of …, 2017 - spiedigitallibrary.org
… performing fluorescence spectroscopy and fluorescence lifetime measurements in clinics,
its characterization, and its validation with measurements in biological tissues. Normal tissue, …

Fluorescence lifetime techniques in medical applications

L Marcu - Annals of biomedical engineering, 2012 - Springer
… of time-resolved (lifetime) fluorescence techniques used in … fluorescence spectroscopy
(TRFS) and fluorescence lifetime … and diagnosis of biological tissues. Emphasis is placed on the …

Fluorescence lifetime imaging of unstained tissues: early results in human breast cancer

PJ Tadrous, J Siegel, PMW French… - The Journal of …, 2003 - Wiley Online Library
Fluorescence lifetime imaging (FLIM) depends on the fluorescence decay differences between
tissues to generate image contrast. In the present study FLIM has been applied to fixed (…

NAD (P) H fluorescence lifetime measurements in fixed biological tissues

JV Chacko, KW Eliceiri - … and Applications in Fluorescence, 2019 - iopscience.iop.org
… We do observe a change in the fluorescence lifetime and fluorescence spectral signature
with fixation, but the relative changes between any two samples in the NAD(P)H lifetime

Methods for imaging the structure and function of living tissues and cells: 2. Fluorescence lifetime imaging

PJ Tadrous - The Journal of pathology, 2000 - Wiley Online Library
… The passage of excitation and fluorescent light through multiply scattering or heterogeneous
media (such as turbid biological tissues) can be described by the diffusion approximation to …