… scanning microscope combining optical coherence tomography, Raman spectroscopy and fluorescence lifetime microscopy for mesoscale label-free imaging of tissue

D Vasquez, F Knorr, F Hoffmann, G Ernst… - Analytical …, 2021 - ACS Publications
… The present implementation allows the investigation of biological tissues in the mesoscale
range, 0.1–5 mm in a correlated manner. Due to the co-registered acquisition of the modalities…

Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell

PIH Bastiaens, A Squire - Trends in cell biology, 1999 - cell.com
… as fluorescence resonance energy transfer (FRET). These properties of fluorescence lifetimes
… Imaging of fluorescence lifetimes enables biochemical reactions to be followed at each …

Rapid fluorescence lifetime estimation with modified phasor approach and Laguerre deconvolution: a comparative study

F Fereidouni, D Gorpas, D Ma… - … in fluorescence, 2017 - iopscience.iop.org
Fluorescence lifetime imaging has been shown to serve as a valuable tool for … biological
tissue at a mesoscopic level. The ability to analyze fluorescence decay curves to extract lifetime

Effect of fixation and mounting on fluorescence lifetime of cellular autofluorescence

X Wang, Y Xie, M Huang, L Yao, Y Wang… - IEEE Journal of …, 2019 - ieeexplore.ieee.org
… to fresh excision tissues or biopsies. Fixation and mounting is a routine procedure in cell
biology and pathological research [14], which provides good tissue and protein preservation for …

Multiphoton laser microscopy and fluorescence lifetime imaging for the evaluation of the skin

S Seidenari, F Arginelli, S Bassoli… - Dermatology …, 2012 - Wiley Online Library
… of intrinsic tissue fluorophores and nonlinear harmonic generation from tissue matrix …
structural imaging of unstained biological tissue [1–10]. Whereas, for conventional confocal …

Real time optical biopsy: time-resolved fluorescence spectroscopy instrumentation and validation

DS Kittle, F Vasefi, CG Patil, A Mamelak, KL Black… - Scientific reports, 2016 - nature.com
… Most of these fluorophores have a fluorescence lifetime … emission spectra of biological
tissue fluorophores are normally … of tissue, we show that the fluorescence lifetime information is …

Tissue fluorophores and their spectroscopic characteristics

A Chorvatova, D Chorvat - Fluorescence lifetime spectroscopy …, 2014 - api.taylorfrancis.com
… , spectrally resolved fluorescence lifetime analysis of endogenous fluorescence facilitates
even better recognition, discrimination, and diagnosis of biologically active tissues and their …

Fluorescence lifetime imaging from time resolved measurements using a shape-based approach

D Alvarez, P Medina, M Moscoso - Optics Express, 2009 - opg.optica.org
… carried out previously for imaging the fluorescence decay rate map within biological tissue.
… but distinct fluorescence lifetimes between 0.5 ns and 1 ns. To model tissue autofluorescence …

Recent trends in two-photon auto-fluorescence lifetime imaging (2P-FLIM) and its biomedical applications

H Ranawat, S Pal, N Mazumder - Biomedical Engineering Letters, 2019 - Springer
… of the fluorescence lifetime of NADH has been carried out in a variety of biological samples,
… ideal Krogh tissue cylinder exhibits an elemental relationship to tissue oxygen diffusion. The …

Mathematical model for time-resolved and frequency-domain fluorescence spectroscopy in biological tissues

MS Patterson, BW Pogue - Applied Optics, 1994 - opg.optica.org
… the use of the fluorescence lifetime as a diagnostic tool. … ) the fluorescence lifetime can be
perturbed by multiple scattering of both the excitation and the emission light in the host tissue. …