Effects of fetal calf serum, amino acids, vitamins and insulin on blastocoel formation and hatching of in vivo and IVM/IVF-derived porcine embryos developing in vitro

DB Koo, NH Kim, HT Lee, KS Chung - Theriogenology, 1997 - Elsevier
… fetal calf serum (FCS), nonessential MEM amino acids, MEM vitamins and insulin on blastocoel
formation, … (P < 0.05) decreased by the compaction and blastocoel formation of I- to Z-cell …

Effect of fetal calf serum, non essential amino acids and vitamins on the blastocoel formation, expansion and hatching in porcine embryos developing in vitro

HT Lee, DB Koo, JY Ju, NH Kim, KS Chung - Theriogenology, 1997 - infona.pl
calf serum (FCS), 2 MEM non-essential amino acids (AAs) and 2 MEM vitamins (Vits) on the
blastocoel formation… significantly decreased the compaction and blastocoel formation of 1- to …

Effects of Serum on Swine Morulae and Blastocysts in Vitro

JM Robl, DL Davis - Journal of Animal Science, 1981 - academic.oup.com
… containing glucose and bovine serum albumin or in mKRB supplemented with serum. The
… about the time of blastocoel formation, since mKRB will not support development of day 8 …

A serum-free, cell-free culture system for development of bovine one-cell embryos up to blastocyst stage with improved viability

M Shamsuddin, B Larsson, H Gustafsson… - Theriogenology, 1994 - Elsevier
… by the evidence of formation of a distinct blastocoel or by expansion and hatching … calf serum
in medium when the serum was not heat inactivated (23). We have used estrous cow serum

Metabolic characterization of the bovine blastocyst, inner cell mass, trophectoderm and blastocoel fluid

N Gopichandran, HJ Leese - Reproduction, 2003 - rep.bioscientifica.com
formation of the blastocyst in which the outer trophectoderm cells surround the blastocoel
for 2–3 h to recover before incubation in anti-bovine serum (B8270; 10% (v/v) in Hepes-SOF…

[PDF][PDF] Kinetics of early in vitro development of bovine in vivo-and in vitro-derived zygotes produced and/or cultured in chemically defined or serum-containing media

P Holm, PJ Booth, H Callesen - REPRODUCTION-CAMBRIDGE-, 2002 - academia.edu
… This finding indicates that the effect of serum may be linked to … blastocoel formation within
the fifth cell cycle of the bovine embryo rather than to the mechanisms involved in blastocoel

Bovine embryo culture in the presence or absence of serum: implications for blastocyst development, cryotolerance, and messenger RNA expression

D Rizos, A Gutiérrez-Adán… - Biology of …, 2003 - academic.oup.com
… bars) or absence (white bars) of fetal calf serum (FCS). The mean of the four replicates was
… of IFN-τ production is tightly linked to blastocoel formation. IFN-τ can be readily detected in …

… of insulin, transferrin and selenium and epidermal growth factor on development of buffalo oocytes to the blastocyst stage in vitro in serum‐free, semidefined media

HM Raghu, SM Reddy, S Nandi - Veterinary record, 2002 - Wiley Online Library
… was studied in serum-free, semidefined media containing bovine serum albumin, follicle-…
An embryo was classified as a blastocyst upon the formation of a blastocoel, thinning of the …

Proteomic analysis of bovine blastocoel fluid and blastocyst cells

PL Jensen, ML Grøndahl, HC Beck… - Systems Biology in …, 2014 - Taylor & Francis
… In an elegant study, Pedersen and Spindle injected donor 8-cells and morulas into the
blastocoel cavity of giant chimeric blastocysts formed by the aggregation of 8–10 embryos at the 4…

Blastocoel volume is related to successful establishment of human embryonic stem cell lines

EH Cheng, W Chen, SY Chang, JJ Huang… - Reproductive …, 2008 - Elsevier
… consisting of DMEM supplemented with 10% fetal bovine serum (Invitrogen Corp.), 2 mmol/l
L-… (b) Histological analysis of differentiated elements found in teratomas formed in the leg of …