Multiplex PCR for detection of acquired carbapenemase genes

L Poirel, TR Walsh, V Cuvillier, P Nordmann - Diagnostic microbiology and …, 2011 - Elsevier
… By contrast, all the 50 non–carbapenemase-producing enterobacterial … the multiplex PCR
technique is a fast (<4 h) and reliable technique for rapid screening of carbapenemase genes. …

[HTML][HTML] Multi-centre evaluation of real-time multiplex PCR for detection of carbapenemase genes OXA-48, VIM, IMP, NDM and KPC

A van der Zee, L Roorda, G Bosman, AC Fluit… - BMC infectious …, 2014 - Springer
multiplex PCR assay was evaluated using a collection of 86 Gram negative isolates, including
62 carbapenemase … detection of the carbapenemase genes on a selection of 20 isolates. …

Rapid detection of carbapenemase genes by multiplex real-time PCR

J Monteiro, RH Widen, ACC Pignatari… - Journal of …, 2012 - academic.oup.com
multiplex PCR melting curves of the amplicons generated by primers targeting the six
carbapenemases … In summary, the multiplex real-time PCR assay developed in this study is a fast (…

[HTML][HTML] Multiplex PCR for rapid detection of genes encoding class A carbapenemases

SS Hong, K Kim, JY Huh, B Jung… - Annals of laboratory …, 2012 - synapse.koreamed.org
multiplex PCR assay to detect and differentiate CAC genes in … multiplex PCR detected all
genes tested for 4 CAC families that could be differentiated by fragment size according to gene

Novel multiplex PCRs for detection of the most prevalent carbapenemase genes in Gram-negative bacteria within Germany

M Cerezales, L Biniossek, S Gerson… - Journal of Medical …, 2021 - microbiologyresearch.org
multiplex PCR for the rapid and reliable identification of the most prevalent
carbapenemase-encoding genes … revealed the most prevalent carbapenemase groups in …

[HTML][HTML] Multiplex real-time PCR assay for six major carbapenemase genes

N Yoshioka, H Hagiya, M Deguchi, S Hamaguchi… - Pathogens, 2021 - mdpi.com
multiplex assay to detect six major carbapenemase genes using previously developed single
PCR … A recent study has also described a multiplex PCR assay using LightCycler ® 480 II (…

Development and evaluation of multiplex PCR for the detection of carbapenemase-producing Enterobacteriaceae

SH Kim, IK Bae, NY Kim, S Am Song… - Annals of Clinical …, 2019 - synapse.koreamed.org
… several important CPE genes simultaneously. Methods: We aimed to develop a multiplex
PCR that could detect seven CPE genes simultaneously. The multiplex PCR was composed of …

Development of a set of multiplex PCR assays for the detection of genes encoding important β-lactamases in Enterobacteriaceae

C Dallenne, A Da Costa, D Decré… - Journal of …, 2010 - academic.oup.com
… None of the carbapenemase genes targeted was found in this study. Reduced susceptibility
to imipenem found in one K. pneumoniae isolate was probably due to a decrease in …

[HTML][HTML] … Characterization of Acinetobacter baumannii Clones with rep-PCR and Evaluation of Carbapenemase Genes by New Multiplex PCR in Hospital District of …

T Pasanen, S Koskela, S Mero, E Tarkka, P Tissari… - PloS one, 2014 - journals.plos.org
… to reliably exclude carbapenemase genes. The aim of this study was to investigate the
carbapenemase genes of A. baumannii and the correlation between these genes and clonal …

Molecular characterization of carbapenemase producing Klebsiella pneumoniae strains by multiplex PCR and PFGE methods: The first K. pneumoniae isolates co …

S Genç, F Kolaylı, EY Özçelik - Journal of Infection and Chemotherapy, 2022 - Elsevier
… of transferable carbapenemase genes of carbapenem resistant K. … one carbapenemase
gene except bla IMP was determined in 95 of 100 isolates, none of the carbapenemase genes