… cryo-FIB and cryo-transmission electron microscope (cryo-TEM). Here, we describe our
optimized protocol and modified instrumentation for cryo-FIB … into our cartridge-type cryo-TEM. …

… been reported demonstrating the cryo-FIB lift-out procedure, … preparation of cryo-TEM
lamellae using cryo-FIB and show … the materials scientist through the cryo-FIB lift-out process, …

… Cryo-Lift-Out (cryo-LO) technique as preparation tool for cryogenic transmission electron
microscopy (cryo… key considerations for the practical usage of cryogenic Lift-Out for those new to …

… popular type of high-end cryo-TEM, having a cartridge-type specimen holder. In that case,
the cartridge itself is modified for use in both the FIB-SEM and the TEM (Kuba et al., 2020). …

… To address these problems, we have designed a sample cryo-… cartridges to simplify the
transfer process between FIB and TEM. We optimized several parameters in the cryo-FIB and cryo…

… Our workflow was developed in an effort to establish cryo-FIB … For the Polara, a modified
cartridge that accepted an … the Titan Krios, or regular sample cartridges for imaging in the Polara. …

… (J) The cryo-stage holder for the cartridge in the cryo-FIB, featuring an open … cryo-stage
holder is positioned on the rod to facilitate the cryo-transfer step from the cryo-FIB/SEM to the cryo-…

… AMBER cryo FIB-SEM microscope equipped with a Leica VCT500 cryo-transfer and cryo-…
and can be used routinely for cartridge-based or side-entry cryo TEM stages. Moreover, the …

… Here, we detail the steps needed to generate lamellae samples using cryo-FIB milling of …
with Autoloader, or Polara microscope with the modified cartridge or modified side entry holder …

… cryo-stage and a VCT500 cryo-transfer system. Grids were clipped in autogrid cartridges prior
to loading into the cryo-FIB… standard operating procedures for cryo-FIB milling, accordingly, …