Amino acid metabolism in mammalian cell cultures

H Eagle - Science, 1959 - science.org
… with the amino acid metabolism of these cultured cells, is a … amino acids required by
long-term cell cultures were necessary also for the survival and growth of these first-passage cells

[HTML][HTML] Amino acids in the cultivation of mammalian cells

A Salazar, M Keusgen, J Von Hagen - Amino acids, 2016 - Springer
… Studying amino acids in mammalian cell culture media will help provide a better … Cellular
fate of amino acids in cell culture. a Comparison of the amino acid composition of the CHO-K1 …

Amino acids and peptide‐based supramolecular hydrogels for three‐dimensional cell culture

XQ Dou, CL Feng - Advanced Materials, 2017 - Wiley Online Library
cells into hydrogels for 3D culture. This review will assess current progress in the preparation
of amino acids … stimuli, and in situ encapsulation of cells into the hydrogels, with a focus on …

Stable isotope labeling by amino acids in cell culture for quantitative proteomics

SE Ong, B Blagoev, I Kratchmarova, LJ Foster… - Cell Biology, 2006 - Elsevier
… lines with growth media containing less arginine or lysine, we find that significantly
reducing the amount of these amino acids does not adversely affect the growth of cells. …

Optimizing amino acid composition of CHO cell culture media for a fusion protein production

Z Xing, B Kenty, I Koyrakh, M Borys, SH Pan, ZJ Li - Process Biochemistry, 2011 - Elsevier
… limiting amino acids were increased while those in excess were reduced to obtain balanced
amino acid composition in both the batch and feed media. The fed-batch cell cultures using …

A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC)

SE Ong, M Mann - Nature protocols, 2006 - nature.com
Cells grown in this medium incorporate the heavy amino acids after five cell doublings and
SILAC amino acids have no effect on cell morphology or growth rates. When light and heavy …

[HTML][HTML] Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics

SE Ong, B Blagoev, I Kratchmarova… - Molecular & cellular …, 2002 - ASBMB
acids in cell culture). Labeled, essential amino acids are added to amino acid deficient cell
culture … is compatible with virtually all cell culture conditions, including primary cells. We show …

Quantitative proteomics using stable isotope labeling with amino acids in cell culture

HC Harsha, H Molina, A Pandey - Nature protocols, 2008 - nature.com
… on ensuring heavy amino acid incorporation in the cell population. … cells to avoid free amino
acids that are potentially present in the serum. Although this has worked well for several cell

Use of stable isotope labeling by amino acids in cell culture as a spike-in standard in quantitative proteomics

T Geiger, JR Wisniewski, J Cox, S Zanivan, M Kruger… - Nature protocols, 2011 - nature.com
cell culture with heavy forms of essential amino acids. Dialyzed serum is used so that the
added amino acids … In contrast to labeling with heavy nitrogen or carbon, the heavy amino acids

THE SPECIFIC AMINO ACID REQUIREMENTS OF A HUMAN CARCINOMA CELL (STRAIN HELA) IN TISSUE CULTURE

H Eagle - The Journal of experimental medicine, 1955 - rupress.org
… describes the amino acid requirements of a human uterine epithelial carcinoma cell (strain …
species and in cell type, the amino acid requirements of the two cell lines were qualitatively …