Fundamentals of microfluidic cell culture in controlled microenvironments

EWK Young, DJ Beebe - Chemical Society Reviews, 2010 - pubs.rsc.org
… key elements of microscale cell culture and the major … cell microenvironment, discuss
conventional cell culture techniques, and summarize the major techniques for microfluidic control

Designing microgels for cell culture and controlled assembly of tissue microenvironments

AS Caldwell, BA Aguado… - Advanced functional …, 2020 - Wiley Online Library
cell microenvironments. The microgels can function as either individual cell culture units or
… allows user‐defined control of the microenvironments, from the order of singly encapsulated …

Design and development of microbioreactors for long-term cell culture in controlled oxygen microenvironments

HE Abaci, R Devendra, Q Smith, S Gerecht… - Biomedical …, 2012 - Springer
… are exposed in tissue culture … -term cell culture studies with the capability to accurately
control and continuously monitor the dissolved oxygen (DO) level in the cell microenvironment. In …

Review of microfluidic cell culture devices for the control of gaseous microenvironments in vitro

HM Wu, TA Lee, PL Ko, HJ Chiang… - Journal of …, 2018 - iopscience.iop.org
… cellular gaseous microenvironments have been proposed in recent years to overcome
the … cell culture devices that have been developed to control gaseous microenvironments

Engineered microenvironments for controlled stem cell differentiation

JA Burdick, G Vunjak-Novakovic - Tissue Engineering Part A, 2009 - liebertpub.com
… Recent studies of cancer cells in 3D cell culture … to control features at length scales from 1
μm to 1 cm 97 and are compatible with cells to control the cellular microenvironment in culture

Engineering cellular microenvironments with photo-and enzymatically responsive hydrogels: toward biomimetic 3D cell culture models

RY Tam, LJ Smith, MS Shoichet - Accounts of chemical research, 2017 - ACS Publications
… (3) While this early work clearly demonstrated our ability to control cell function in specific
volumes in a 3D hydrogel, peptide immobilization could only be achieved as a uniform column …

Optimizing design and fabrication of microfluidic devices for cell cultures: An effective approach to control cell microenvironment in three dimensions

G Pagano, M Ventre, M Iannone, F Greco… - …, 2014 - pubs.aip.org
… Stem Cells under the effects of Bone Morphogenetic Protein-2 gradients were performed
in order to demonstrate the efficacy of the system in controlling cell microenvironment. The …

[HTML][HTML] Methods to study the tumor microenvironment under controlled oxygen conditions

MB Byrne, MT Leslie, HR Gaskins, PJA Kenis - Trends in biotechnology, 2014 - cell.com
… media for cells, requires the cell culture flask to be removed from a gas-controlled incubator
… laboratory techniques quickly becomes arduous under controlled oxygen concentrations. …

Cell patterning chip for controlling the stem cell microenvironment

A Rosenthal, A Macdonald, J Voldman - Biomaterials, 2007 - Elsevier
… exquisite control of the cellular microenvironment, we … cell culture techniques control the
cell density only at the macroscopic level of the cell-culture dish, creating poor uniformity in cell

Engineered culture models for studies of tumor-microenvironment interactions

DW Infanger, ME Lynch… - Annual review of …, 2013 - annualreviews.org
… Traditional cell culture approaches are restricted in their ability … microenvironmental conditions.
Specifically, tissue spreading is controlled via the coordinated interplay between cell-cell