Hydrogen peroxide. Ubiquitous in cell culture and in vivo?

B Halliwell, MV Clement, J Ramalingam, LH Long - IUBMB life, 2000 - Wiley Online Library
Cell culture is a widely used method for the investigation of “physiological” … involving
cell culture media are rarely considered. Addition of reducing agents to commonly used cell-culture

Cell culture models for oxidative stress: superoxide and hydrogen peroxide versus normobaric hyperoxia

JJP Gille, H Joenje - Mutation Research/DNAging, 1992 - Elsevier
… In cells, the deleterious effects of activated oxygen species … Cell culture systems are
especially useful to study the effects … as observed in cultures of Chinese hamster cell lines. Since …

Instability of, and generation of hydrogen peroxide by, phenolic compounds in cell culture media

LH Long, A Hoi, B Halliwell - Archives of Biochemistry and Biophysics, 2010 - Elsevier
… Loss of compounds in cell culture media was measured using an HPLC system … cell culture
medium to the required final concentrations in a 24-well plate and incubated in a cell culture

Assessment of cell death studies by monitoring hydrogen peroxide in cell culture

I Hirsch, E Prell, M Weiwad - Analytical biochemistry, 2014 - Elsevier
… of cell populations according to the FACS analysis. The percentage of cells was plotted against
corresponding hydrogen peroxide … of H 2 O 2 concentrations in cell culture medium. This …

Hydrogen peroxide production is affected by oxygen levels in mammalian cell culture

LA Maddalena, SM Selim, J Fonseca… - Biochemical and …, 2017 - Elsevier
Hydrogen peroxide in particular participates in … cell culture conditions is important to
understand. We measured the rates of cellular hydrogen peroxide production in some common cell

Oxidation and generation of hydrogen peroxide by thiol compounds in commonly used cell culture media

LH Long, B Halliwell - Biochemical and Biophysical Research …, 2001 - Elsevier
… cysteamine, homocysteine) were found to interact with three commonly used cell culture
media (RPMI, MEM, DMEM) to generate hydrogen peroxide with complex concentration-…

A detailed analysis of hydrogen peroxide-induced cell death in primary neuronal culture

ER Whittemore, DT Loo, JA Watt, CW Cotmans - Neuroscience, 1995 - Elsevier
… apoptotic cell suicide pathway. The studies reported here investigate H 2 O 2 -induced cell
… 2 O 2 can induce apoptotic cell death within 3 h, as assessed by cell viability, morphological …

… cell culture: rapid generation of hydrogen peroxide on addition of (−)-epigallocatechin,(−)-epigallocatechin gallate,(+)-catechin, and quercetin to commonly used cell …

LH Long, MV Clement, B Halliwell - Biochemical and Biophysical Research …, 2000 - Elsevier
… used cell culture media leads to generation of substantial amounts of hydrogen peroxide (…
We recommend that in cell culture studies using DMEM, RPMI, McCoy and probably other …

Formation of hydrogen peroxide in cell culture media by apple polyphenols and its effect on antioxidant biomarkers in the colon cell line HT‐29

P Bellion, M Olk, F Will, H Dietrich… - Molecular nutrition & …, 2009 - Wiley Online Library
hydrogen peroxide (H2O2) formation was determined after incubation of apple juice extracts
(AEs) and polyphenols in cell culture … ) level of HT-29 cells were studied by coincubation l …

Artefacts in cell culture: pyruvate as a scavenger of hydrogen peroxide generated by ascorbate or epigallocatechin gallate in cell culture media

LH Long, B Halliwell - Biochemical and biophysical research …, 2009 - Elsevier
… compounds readily oxidise in cell culture media to generate hydrogen peroxide. However, …
under test can sometimes readily oxidise in cell culture media, that this might not be detected …