… , we recommend SILAC in cell culture systems. Chemical labeling … cell culture models and
adapt the approach for their specific needs. In our protocol, we describe the adaptation of cells …

… , cell culture was still largely a research tool with a relatively small following. There was still
an element of distrust that cell culture … materials sections of the protocols and the recipes for …

… and cells. In this paper, we review current examples of performance-based hydrogel design
for 3D cell culture … are being adapted to analysis of 3D cell culture in hydrogel systems. …

… When purchasing commercial kits or following specific hydrogel recipes, these may already
be known … and how they may influence the utility of hydrogels for cell culture applications. …

… and Carrel improved Harrison's cell cultures. In mid-… animal cell cultures in vitro were
developed, and human diploid cell lines were established. On the basis of knowledge about the cell …

… cells that maintains their health and sterility for many months. Using conventional techniques,
primary neuron cultures … decline in the health of these cultures. Because of this and the ever…

… Add complete medium to the tube containing the cells and place the cells in cell culture …
Use deionized, distilled water in all recipes and protocol steps. For common stock solutions, …

… that provide excellent recipe books for molecular techniques. Practical cell biology normally
has a much lower profile, usually with a few more theoretical books on the cell types being …

… First, presentation in a recipe format, in which exact details for specific cell types are given, is
… The object of tissue culture is to maintain viable single cells (cell culture) or a functional unit …

… They contain recipes for the formulation of mTeSR (a defined medium for human ES cell
culture) and detailed protocols for the culture, transfer, and passage of cells grown in these …