Stable isotope labeling with amino acids in cell culture (SILAC) for studying dynamics of protein abundance and posttranslational modifications
… for investigating the dynamics of protein abundance and posttranslational modifications. … to
study protein complexes, protein-protein interactions, and the dynamics of protein abundance …
study protein complexes, protein-protein interactions, and the dynamics of protein abundance …
Insights into the regulation of protein abundance from proteomic and transcriptomic analyses
C Vogel, EM Marcotte - Nature reviews genetics, 2012 - nature.com
… abundances only partially predict protein abundances 1 , … how the levels of proteins are set
within cells. In this Progress article… in bacteria, yeast and human cell culture: beyond a certain …
within cells. In this Progress article… in bacteria, yeast and human cell culture: beyond a certain …
Considerations when quantitating protein abundance by immunoblot
AA McDonough, LC Veiras… - … of Physiology-Cell …, 2015 - journals.physiology.org
… for quantitative analysis of protein abundance: 1) Consider … abundance. 2) Establish the
specificity of the antiserum for the … , but the same considerations apply to cell culture samples. …
specificity of the antiserum for the … , but the same considerations apply to cell culture samples. …
Stable isotope labeling by amino acids in cell culture based proteomics reveals differences in protein abundances between spiral and coccoid forms of the gastric …
… improve accuracy in detecting protein abundance changes in H. pylori strain 26695. Applying
this method, we investigated distinct differences in protein abundance between the spiral …
this method, we investigated distinct differences in protein abundance between the spiral …
[HTML][HTML] Protein abundance profiling of the Escherichia coli cytosol
… coli proteins presented in this work represent the most complete study … protein abundance
in a bacterial cell so far. We show significant associations between the abundance of a protein …
in a bacterial cell so far. We show significant associations between the abundance of a protein …
Sequence signatures and mRNA concentration can explain two‐thirds of protein abundance variation in a human cell line
… of translation and protein degradation on protein abundance variation in a human cell line.
For this purpose, we experimentally measured absolute protein and mRNA concentrations in …
For this purpose, we experimentally measured absolute protein and mRNA concentrations in …
[HTML][HTML] On the dependency of cellular protein levels on mRNA abundance
… ; pSILAC, pulsed stable isotope labeling by amino acids in cell culture; PUNCH-P, puromycin-…
Protein Abundance Conventionally, the measurement of protein abundances in biological …
Protein Abundance Conventionally, the measurement of protein abundances in biological …
Effects of cell culture media on the dynamic formation of protein− nanoparticle complexes and influence on the cellular response
G Maiorano, S Sabella, B Sorce, V Brunetti… - ACS …, 2010 - ACS Publications
… composition of protein corona does not reflect the relative abundance of serum proteins. To
… , several comparative viability assays onto two cell lines (HeLa and U937) were carried out …
… , several comparative viability assays onto two cell lines (HeLa and U937) were carried out …
[HTML][HTML] … modified protein abundance index (emPAI) for estimation of absolute protein amount in proteomics by the number of sequenced peptides per protein* s
Y Ishihama, Y Oda, T Tabata, T Sato, T Nagasu… - Molecular & Cellular …, 2005 - ASBMB
… the PAI strategy to determine protein abundance from nano-LC-MS… emPAI to be roughly
proportional to protein abundance. … Stable isotope labeling by amino acids in cell culture, SILAC, …
proportional to protein abundance. … Stable isotope labeling by amino acids in cell culture, SILAC, …
[HTML][HTML] Identification of secreted proteins by comparison of protein abundance in conditioned media and cell lysates
PS Abbineni, VT Tang, F da Veiga Leprevost… - Analytical …, 2022 - Elsevier
… proteins are relatively more abundant in the cell conditioned media relative to the cell lysate.
… Analysis of the full spectrum of secreted proteins in cell culture is complicated by leakage of …
… Analysis of the full spectrum of secreted proteins in cell culture is complicated by leakage of …
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