Preparation of cell cultures and vaccinia virus stocks

CA Cotter, PL Earl, LS Wyatt… - Current Protocols in …, 2017 - Wiley Online Library
… modified vaccinia virus Ankara (MVA) strain of vaccinia virus. Protocols for the preparation,
titration, and trypsinization of vaccinia virus stocks, as well as viral DNA preparation and virus

Clonal vaccinia virus grown in cell culture as a new smallpox vaccine

R Weltzin, J Liu, KV Pugachev, GA Myers, B Coughlin… - Nature medicine, 2003 - nature.com
vaccinia virus that was derived from the existing Dryvax vaccine for growth in a human diploid
cell … and to induce neutralizing antibody and cell-mediated immunity against vaccinia virus. …

Preparation of cell cultures and vaccinia virus stocks

PL Earl, N Cooper, LS Wyatt, B Moss… - Current Protocols in …, 1998 - Wiley Online Library
… the maintenance of cell lines used with vaccinia virus, both in monolayer cultures and in
suspension. The suspended cell culture is then used in the preparation of vaccinia virus stocks. …

The purification of vaccinia virus from cell cultures

DN Planterose, C Nishimura, NP Salzman - Virology, 1962 - Elsevier
Vaccinia virus has been grown in HeLa cell suspension and purified by fluorocarbon
treatment. Cellular … With this strain of virus and the cell culture used, newly formed virus is not …

Superinfection exclusion of vaccinia virus in virus-infected cell cultures

L Christen, J Seto, EG Niles - Virology, 1990 - Elsevier
… that vaccinia virus is … cells within 4 hr after the onset of the initial virus infection. Furthermore,
we show that replication of the superinfecting virus is inhibited at a point between the virus

[HTML][HTML] ACAM2000 clonal Vero cell culture vaccinia virus (New York City Board of Health strain)–a second-generation smallpox vaccine for biological defense

TP Monath, JR Caldwell, W Mundt, J Fusco… - International journal of …, 2004 - Elsevier
viruses and in light of advances in technology. Since vaccinia virus grows to high titers in a
variety of cell culture … in an approved cell bank, preferably in continuous cells that had been …

[HTML][HTML] Vaccinia virus strain differences in cell attachment and entry

Z Bengali, AC Townsley, B Moss - Virology, 2009 - Elsevier
Vaccinia virus (VACV) strain WR can enter cells by a low pH endosomal pathway or direct
… of isolates of other orthopoxviruses that have not been passaged extensively in cell culture. …

K+ channel expression in primary cell cultures mediated by vaccinia virus

A Karschin, J Alyar, A Gouin, N Davidson… - FEBS …, 1991 - Wiley Online Library
… We report here on the extension ot this system to postmitotic mar' malian cells m primary
culture. … express,on of Drosophila Shaker H4 K* channels m primary cell cultures a Host cell

… deletions of modified vaccinia virus Ankara (MVA) into the parental vaccinia virus is not sufficient to reproduce an MVA-like phenotype in cell culture and in mice

C Meisinger-Henschel, M Späth, S Lukassen… - Journal of …, 2010 - Am Soc Microbiol
… Modified vaccinia virus Ankara (MVA) has a highly restricted host range in cell culture
and is apathogenic in vivo. MVA was derived from the parental chorioallantois vaccinia virus

Activity of distamycin A on vaccinia virus infection of cell cultures

MA Verini, M Ghione - Chemotherapy, 1964 - karger.com
… other viruses (ie Polyoma virus, Newcastle Disease Virus (NDV… the infection of cell cultures
by vaccinia virus. Other authors (… this antibiotic on DNA virus in various experimental systems. …