Neurosphere and neurosphere-forming cells: morphological and ultrastructural characterization

A Bez, E Corsini, D Curti, M Biggiogera, A Colombo… - Brain research, 2003 - Elsevier
… are not synchronized and are represented in all phases of the cell cycle. Cytofluorimetric …
that human neurospheres are highly dynamic structures with distinct radial gradients of cell

Generation of neurospheres from human adipose‐derived stem cells

E Yang, N Liu, Y Tang, Y Hu, P Zhang… - BioMed research …, 2015 - Wiley Online Library
… into various kinds of cells; this study intends to generate neurospheres from human ADSCs
by culturing ADSCs on … The cell cycle analyses of neurospheres showed 15.38% of cells in …

Effect of neurosphere size on the growth rate of human neural stem/progenitor cells

H Mori, K Ninomiya, M Kino‐oka… - Journal of …, 2006 - Wiley Online Library
… free cell cluster that permits the proliferation and/or maintenance of cellular properties. … of
human NSPCs and examined the human neurosphere formation process, focusing on the cell

Human neurospheres as three-dimensional cellular systems for developmental neurotoxicity testing

M Moors, TD Rockel, J Abel, JE Cline… - Environmental …, 2009 - ehp.niehs.nih.gov
… Among all stained cells, 97.75% showed only a G 0 /G 1 peak (Figure 3F), whereas we found
a typical cell cycle distribution for proliferating cells in only 2.72% of the population. About …

Cell cycle kinetics of expanding populations of neural stem and progenitor cells in vitro

S Alam, A Sen, LA Behie… - Biotechnology and …, 2004 - Wiley Online Library
… Second, it was observed that different culturing methods influence both the proportion of
neurosphere cells in each phase of the cell cycle and the fraction of actively proliferating …

Slower cycling of nestin-positive cells in neurosphere culture

BF Ma, XM Liu, XM Xie, AX Zhang, JQ Zhang… - …, 2006 - journals.lww.com
… ], suggesting that the cell cycle is different for different types of cells in vivo. Cell cycle plays
an important … and maintenance of stem cells [ 8 ]. So far, many important questions about the …

Scientific validation of human neurosphere assays for developmental neurotoxicity evaluation

K Koch, K Bartmann, J Hartmann, J Kapr… - Frontiers in …, 2022 - frontiersin.org
… In this study, we scientifically validate the Neurosphere Assay, which is based on human
primary neural progenitor cells (hNPCs) and an integral part of the DNT IVB. It assesses …

Optimal time for passaging neurospheres based on primary neural stem cell cultures

F Xiong, H Gao, Y Zhen, X Chen, W Lin, J Shen, Y Yan… - Cytotechnology, 2011 - Springer
… Proliferating cells traverse the cell cycle from one mitosis to … in the initiation and progression
of the cell cycle. Cyclin D1 is … on cellular metabolic activity, cell cycle distribution and related …

Ethanol induces cell-cycle activity and reduces stem cell diversity to alter both regenerative capacity and differentiation potential of cerebral cortical neuroepithelial …

DR Santillano, LS Kumar, TL Prock, C Camarillo… - BMC neuroscience, 2005 - Springer
… Because we observed that ethanol increased cell cycle activity in neurosphere cultures, we
hypothesized that ethanol would also increase telomerase activity. Surprisingly, ethanol had …

Neurospheres: insights into neural stem cell biology

LS Campos - Journal of neuroscience research, 2004 - Wiley Online Library
… assays highlight the role of cell density in neurosphere formation. Secondary neurosphere
formation assays were done at varying cell densities (from 2,000 cells/well to 1 cell/well) for …