Circulating tumor cell detection in advanced non-small cell lung cancer patients by multi-marker QPCR analysis

LA Devriese, AJ Bosma, MM Van De Heuvel… - Lung Cancer, 2012 - Elsevier
… The aim of this study was to explore circulating tumor cell (CTC) detection in advanced
non-small cell lung cancer (NSCLC). CTCs may not only serve as a prognostic marker in selected …

Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR

T Watanabe, T Miura, Y Degawa, Y Fujita… - Cancer cell …, 2010 - Springer
… lines plus four additional validation lung cancer cell lines (… of lung cancer cell lines using
principal component analysis (PCA) … Our results confirm that qPCR and PCA analysis provide a …

A quantitative analysis of the potential biomarkers of non-small cell lung cancer by circulating cell-free DNA

L Wei, W Wu, L Han, W Yu, Y Du - Oncology Letters, 2018 - spandidos-publications.com
… cfDNA by using real-time PCR with various reference genes … cfDNA of NSCLC patients
quantified by qPCR results using primer … Therefore, we used absolute qPCR analysis of LINE-1 to …

Gene expression profiling reveals novel biomarkers in nonsmall cell lung cancer

A Sanchez‐Palencia, M Gomez‐Morales… - … journal of cancer, 2011 - Wiley Online Library
… the patients analyzed in … qPCR and the robust Bonferroni test to validate the findings (differently
expressed genes) obtained in the microarray analysis in an independent set of patients. …

Exosomal miRNA analysis in non-small cell lung cancer (NSCLC) patients' plasma through qPCR: a feasible liquid biopsy tool

M Giallombardo, JC Borrás, M Castiglia… - JoVE (Journal of …, 2016 - jove.com
… transmission electron microscopy (TEM) analysis, the standard techniques to obtain …
analyzed taking advantage of the remarkable sensitivity and specificity of Real-Time PCR analysis; …

Influence of RT-qPCR primer position on EGFR interference efficacy in lung cancer cells

G Chen, P Kronenberger, E Teugels… - Biological procedures …, 2010 - Springer
… a primer position effect were based upon analysis 24 hrs post treatment, a time point at … cell
line (IMR-32) was also described by the group of Vandesompele [5]. Their RT-qPCR analysis

Rapid KRAS, EGFR, BRAF and PIK3CA Mutation Analysis of Fine Needle Aspirates from Non-Small-Cell Lung Cancer Using Allele-Specific qPCR

R van Eijk, J Licht, M Schrumpf, M Talebian Yazdi… - PloS one, 2011 - journals.plos.org
lung, malignant cells and non small cell lung cancer” and “mediastinum, malignant cells and
non small cell lung cancercases for which tumor-positive histological material of the primary …

Multigene real-time PCR detection of circulating tumor cells in peripheral blood of lung cancer patients

DC Hayes, H Secrist, CS Bangur, T Wang… - Anticancer …, 2006 - ar.iiarjournals.org
… the detection of tumor cells in the peripheral blood of NSCLC and SCLC lung cancer patients.
By … techniques with microarray analyses, our group identified several over-expressed lung

Identification of accurate reference genes for RT-qPCR analysis of formalin-fixed paraffin-embedded tissue from primary non-small cell lung cancers and brain and …

S Søes, BS Sørensen, J Alsner, J Overgaard, H Hager… - Lung Cancer, 2013 - Elsevier
… the cancer metastasize. Using an optimized RT-qPCR workflow we have analyzed the …
Cell Lung Cancer tumors, brain metastases, and lymph node metastases as well as normal …

Selection of suitable reference genes for accurate normalization of gene expression profile studies in non-small cell lung cancer

S Saviozzi, F Cordero, ML Iacono, S Novello… - BMC cancer, 2006 - Springer
… A meta-analysis of lung cancer transcription profiles generated with … conditions are thus
critical issues in qPCR studies [8]. … Most lung cancer qPCR studies use commercial RGs, such as …