A natural inhibitor from Alisma orientale against human carboxylesterase 2: Kinetics, circular dichroism spectroscopic analysis, and docking simulation

J Yi, R Bai, Y An, TT Liu, JH Liang, XG Tian… - International journal of …, 2019 - Elsevier
… for natural human carboxylesterase 2 (human CES 2) inhibitors from traditional Chinese
medicine, we found that the extract of Alisma orientale significantly inhibited human CES 2 in …

Expression and characterization of a human carboxylesterase 2 splice variant

MA Schiel, S Green, WI Davis, PC Sanghani… - … of Pharmacology and …, 2007 - ASPET
Circular dichroism spectra for the proteins were determined using a J-720 spectropolarimeter
(… E, circular dichroism spectrum of CES2 (E2) and CES2Δ 458–473 (V2) performed under …

Structure and conformation of human pancreatic carboxyl‐ester hydrolase

O Guy, D Lombardo, JG Brahms - European Journal of …, 1981 - Wiley Online Library
… The circular dichroism spectrum recorded in the vacuum … If we compare the circular dichroism
data of carboxylester … known from X-ray analysis, human carboxyl-ester hydrolase enters …

Characterization of a novel hyper-thermostable and chlorpyrifos-hydrolyzing carboxylesterase EstC: a representative of the new esterase family XIX

B Wang, S Wu, X Chang, J Chen, J Ma, P Wang… - Pesticide Biochemistry …, 2020 - Elsevier
Carboxylesterases have widely been used in a series of … In the present study, EstC, a novel
carboxylesterase from … Phylogenetic analysis showed that EstC can be assigned as the first …

Investigation of the inhibitory effect of protostanes on human carboxylesterase 2 and their interaction: Inhibition kinetics and molecular stimulations

X Lv, R Bai, JK Yan, HL Huang, XK Huo… - International Journal of …, 2021 - Elsevier
… In this study, the inhibitory activities of a library of protostanes (1–25) against human CES 2
were … for CES have been identified in human body, namely carboxylesterase 1 (CES 1) and …

[HTML][HTML] Efficient in vitro refolding and functional characterization of recombinant human liver carboxylesterase (CES1) expressed in E. coli

U Boonyuen, K Promnares, S Junkree, NPJ Day… - Protein Expression and …, 2015 - Elsevier
… The proteins obtained after refolding were subjected to spectroscopicmethods presented
in this study allow the production of a significant amount as well as a functionally active human

A novel 15-spiro diterpenoid dimer from Andrographis paniculata with inhibitory potential against human carboxylesterase 2

CP Sun, ZJ Yang, WY Zhao, RY Zhang, H Li… - Bioorganic …, 2020 - Elsevier
… 2D NMR, HRESIMS, electronic circular dichroism (ECD), and TD DFT calculations of ECD
spectra. It showed potent inhibitory activity against human carboxylesterase 2 (CES 2) with an …

Demethylbellidifolin isolated from Swertia bimaculate against human carboxylesterase 2: Kinetics and interaction mechanism merged with docking simulations

TT Liu, XK Huo, XG Tian, JH Liang, J Yi, XY Zhang… - Bioorganic …, 2019 - Elsevier
… In this study, forty-nine kinds of traditional Chinese medicines (TCMs) were evaluated for
their inhibitory activities against human carboxylesterase 2 (HCE 2) using a human liver …

Protostane Triterpenoids from the Rhizome of Alisma orientale Exhibit Inhibitory Effects on Human Carboxylesterase 2

ZP Mai, K Zhou, GB Ge, C Wang, XK Huo… - Journal of natural …, 2015 - ACS Publications
methods, including 1D and 2D NMR, HRESIMS, and induced electronic circular dichroism
(IECD… Analyses of the 1 H and 13 C NMR spectroscopic data revealed structural similarities to …

Low-resolution envelope, biophysical analysis and biochemical characterization of a short-chain specific and halotolerant carboxylesterase from Bacillus licheniformis

AM Nakamura, MAS Kadowaki, A Godoy… - International journal of …, 2018 - Elsevier
… [10], carboxylesterases have higher activity against short … Carboxylesterases lack the cap
domain and do not require … Circular Dichroism (CD) spectrum of B. licheniformis BlEst1 as …