… +, dehydrogenases exhibit a pronounced specificity for either one of these coenzymes,
suggesting strict … The directed change in coenzyme specificity of a selected dehydrogenase is an …

… the changes in coenzyme specificity from NAD+ to NADP+ in FDH from baker's yeast [18].
The experiments of changes in FDH coenzyme specificity are described in more detail in [7]. • …

… of coenzyme specificity. Sequence alignment with other formate dehydrogenases revealed
two … ) that could account for the observed coenzyme specificity. Positions 195 and 196 were …

… 1.2, FDH)) with its substrate specificity changed from NAD+ to … of a NAD+-specific formate
dehydrogenase from Saccharomyces … the absolute coenzyme specificity of SceFDH for NAD+. …

… Formate dehydrogenases (FDHs) are frequently used for the regeneration of cofactors in
biotransformations employing NAD(P)H-dependent oxidoreductases. Major drawbacks of most …

… reconstructed with different protein engineering approaches for altering the coenzyme
specificity are outlined. To switch the coenzyme preference of FDHs or to find the native NADP + -…

… FDHs are highly specific towards NADI and do not utilize NADP+ as a coenzyme. However,
at least one of them, FDH-Ps [24], displays dual coenzyme specificity. Under optimal reaction …

… The review summarizes the results on engineering of bacterial and yeast FDHs aimed at
improving their chemical and thermal stability, catalytic activity, switch in coenzyme specificity …

… To confirm the nature of the enzyme as a genuine formate dehydrogenase, we added 1 mM
sodium azide to the reaction mixture. This compound completely inhibited formate oxidation …

… formate dehydrogenase (cmFDH) was constructed based on the Pseudomonas sp. 101
formate dehydrogenase (… a major determinant of coenzyme specificity in NAD/NADP-dependent …