A computational genomics pipeline for prokaryotic sequencing projects

AO Kislyuk, LS Katz, S Agrawal, MS Hagen… - …, 2010 - academic.oup.com
… of high homology and full consensus between four of the … of the pipeline, it involves manual
processing of the assemblyassembly stage with a simple change to the pipeline code. …

Evaluation and validation of de novo and hybrid assembly techniques to derive high-quality genome sequences

SM Utturkar, DM Klingeman, ML Land… - …, 2014 - academic.oup.com
… It should be noted that like HGAP, the PBcR pipeline is also capable of performing self-correction
and non-… To improve the consensus accuracy of PacBio assemblies, we performed …

A post-assembly genome-improvement toolkit (PAGIT) to obtain annotated genomes from contigs

MT Swain, IJ Tsai, SA Assefa, C Newbold… - Nature protocols, 2012 - nature.com
… In this protocol, we address these problems of genome quality through a pipeline of … or
other misassemblies in consensus sequences. Every genome assembly algorithm has a unique …

From cheek swabs to consensus sequences: an A to Z protocol for high-throughput DNA sequencing of complete human mitochondrial genomes

AC Clarke, S Prost, JAL Stanton, WTJ White… - BMC genomics, 2014 - Springer
… associated with the bioinformatics pipeline. The consistent … assembly of the sequencing
reads. In general, two approaches can be used to obtain a consensus sequence or an assembly

Comparative Evaluation of Bioinformatic Pipelines for Full-Length Viral Genome Assembly

L Zsichla, M Zeeb, D Fazekas, E Ay, D Muller… - bioRxiv, 2024 - biorxiv.org
… of consensus genome assembly. In Table 2, we show the comparative performance of the
four pipelines … : in one set, the fixed-reference pipelines were used 266 with the default HXB2 …

A pipeline for the de novo assembly of the Themira biloba(Sepsidae: Diptera) transcriptome using a multiple k-mer length approach

D Melicher, AS Torson, I Dworkin, JH Bowsher - BMC genomics, 2014 - Springer
… Our bioinformatics pipeline uses cloud computing services to assemble and analyze the …
different k-mer assemblies were used to create extended consensus contigs, genes from a …

ViralConsensus: a fast and memory-efficient tool for calling viral consensus genome sequences directly from read alignment data

N Moshiri - Bioinformatics, 2023 - academic.oup.com
pipeline for reconstructing a consensus genome sequence from raw viral amplicon sequence
data is the iVar pipeline (… using Samtools, and a consensus genome sequence is called …

Minimap and miniasm: fast mapping and de novo assembly for noisy long sequences

H Li - Bioinformatics, 2016 - academic.oup.com
assemble 45-fold Caenorhabditis elegans data in 9 min, orders of magnitude faster than the
existing pipelines, though the consensusassembly if we can develop a fast consensus tool …

De novo assembly of highly polymorphic metagenomic data using in situ generated reference sequences and a novel BLAST-based assembly pipeline

YY Lin, CH Hsieh, JH Chen, X Lu, JH Kao, PJ Chen… - … bioinformatics, 2017 - Springer
… , which is independent of reference sequences, possesses the potential to generate a
more complete assembly result including majority consensus sequences and minor variant …

Redundans: an assembly pipeline for highly heterozygous genomes

LP Pryszcz, T Gabaldón - Nucleic acids research, 2016 - academic.oup.com
… Such assemblies mislead downstream analyses, from gene … and homozygous contigs
represent a consensus between two or … back to the assembly, the consensus, homozygous contigs …