Metagenomic assembly through the lens of validation: recent advances in assessing and improving the quality of genomes assembled from metagenomes

ND Olson, TJ Treangen, CM Hill… - … in bioinformatics, 2019 - academic.oup.com
… metagenomic assembly strategies, pipelines and … consensus assembly has high accuracy
exceeding that of short-read data sets because of the signal-based polishing of the consensus

A cloud-compatible bioinformatics pipeline for ultrarapid pathogen identification from next-generation sequencing of clinical samples

SN Naccache, S Federman, N Veeraraghavan… - Genome …, 2014 - genome.cshlp.org
… , the SURPI pipeline continues to the de novo assembly step, … Increased robustness of the
de Brujin graph-based assembly … , along with the final assembled contigs, are subjected to a …

No assembly required: Full-length MHC class I allele discovery by PacBio circular consensus sequencing

CJ Westbrook, JA Karl, RW Wiseman, S Mate… - Human immunology, 2015 - Elsevier
… MHC class I allele discovery approach using circular consensus sequencing (CCS) with
single-… eliminates the ambiguity introduced by assembly of short, highly-similar allele fragments. …

[HTML][HTML] Comparing bioinformatic pipelines for microbial 16S rRNA amplicon sequencing

A Prodan, V Tremaroli, H Brolin, AH Zwinderman… - PLoS …, 2020 - journals.plos.org
pipelines and there is a lack of agreement regarding best practices. Here, we compared six
bioinformatic pipelines … We assessed the sensitivity, specificity, and degree of consensus of …

[HTML][HTML] Genome sequencing of bacteria: sequencing, de novoassembly and rapid analysis using open source tools

V Kisand, T Lettieri - BMC genomics, 2013 - Springer
… through lower than 100% consensus and distracting gene calling due to … Before embarking
on bioinformatic genome analysis, … On this level, all of the pipelines we tested performed very …

The present and future of de novo whole-genome assembly

J Sohn, JW Nam - Briefings in bioinformatics, 2018 - academic.oup.com
assembly step, the reads are assembled as long consensus … also used for the hybrid assembly
pipeline that closes the gaps … The existing hybrid/long-read-only assembly pipelines are …

ToxCodAn-Genome: an automated pipeline for toxin-gene annotation in genome assembly of venomous lineages

PG Nachtigall, AM Durham, DR Rokyta… - …, 2024 - academic.oup.com
… and links to useful resources to learn basic bioinformatics, to build a toxin database from public
… and a consensus quality score (QV) of 36.78, which indicates an accurate assembly with …

SeqyClean: a pipeline for high-throughput sequence data preprocessing

IY Zhbannikov, SS Hunter, JA Foster… - … on bioinformatics …, 2017 - dl.acm.org
… identified while constructing the consensus, the nucleotide call … assembly speed and quality,
requiring the shortest assembly … amount of data to assemble but still preserved enough for …

A bioinformatics pipeline for sequence-based analyses of fungal biodiversity

DL Taylor, S Houston - Fungal Genomics: Methods and Protocols, 2011 - Springer
… Select all the contigs created at the assembly step, then use File → Export → Consensus
Sequences as Single File command, making sure to check the box for “export quality scores” (…

[HTML][HTML] Combining Transcriptome Assemblies from Multiple De Novo Assemblers in the Allo-Tetraploid Plant Nicotiana benthamiana

K Nakasugi, R Crowhurst, J Bally, P Waterhouse - PloS one, 2014 - journals.plos.org
… The general consensus from these studies is that in order to reconstruct a broad range of …
the EvidentialGene tr2aacds pipeline maximises the diversity of de novo assembled transcripts …