Metagenomic assembly through the lens of validation: recent advances in assessing and improving the quality of genomes assembled from metagenomes
… metagenomic assembly strategies, pipelines and … consensus assembly has high accuracy
exceeding that of short-read data sets because of the signal-based polishing of the consensus …
exceeding that of short-read data sets because of the signal-based polishing of the consensus …
A cloud-compatible bioinformatics pipeline for ultrarapid pathogen identification from next-generation sequencing of clinical samples
… , the SURPI pipeline continues to the de novo assembly step, … Increased robustness of the
de Brujin graph-based assembly … , along with the final assembled contigs, are subjected to a …
de Brujin graph-based assembly … , along with the final assembled contigs, are subjected to a …
No assembly required: Full-length MHC class I allele discovery by PacBio circular consensus sequencing
CJ Westbrook, JA Karl, RW Wiseman, S Mate… - Human immunology, 2015 - Elsevier
… MHC class I allele discovery approach using circular consensus sequencing (CCS) with
single-… eliminates the ambiguity introduced by assembly of short, highly-similar allele fragments. …
single-… eliminates the ambiguity introduced by assembly of short, highly-similar allele fragments. …
[HTML][HTML] Comparing bioinformatic pipelines for microbial 16S rRNA amplicon sequencing
A Prodan, V Tremaroli, H Brolin, AH Zwinderman… - PLoS …, 2020 - journals.plos.org
… pipelines and there is a lack of agreement regarding best practices. Here, we compared six
bioinformatic pipelines … We assessed the sensitivity, specificity, and degree of consensus of …
bioinformatic pipelines … We assessed the sensitivity, specificity, and degree of consensus of …
[HTML][HTML] Genome sequencing of bacteria: sequencing, de novoassembly and rapid analysis using open source tools
V Kisand, T Lettieri - BMC genomics, 2013 - Springer
… through lower than 100% consensus and distracting gene calling due to … Before embarking
on bioinformatic genome analysis, … On this level, all of the pipelines we tested performed very …
on bioinformatic genome analysis, … On this level, all of the pipelines we tested performed very …
The present and future of de novo whole-genome assembly
… assembly step, the reads are assembled as long consensus … also used for the hybrid assembly
pipeline that closes the gaps … The existing hybrid/long-read-only assembly pipelines are …
pipeline that closes the gaps … The existing hybrid/long-read-only assembly pipelines are …
ToxCodAn-Genome: an automated pipeline for toxin-gene annotation in genome assembly of venomous lineages
… and links to useful resources to learn basic bioinformatics, to build a toxin database from public
… and a consensus quality score (QV) of 36.78, which indicates an accurate assembly with …
… and a consensus quality score (QV) of 36.78, which indicates an accurate assembly with …
SeqyClean: a pipeline for high-throughput sequence data preprocessing
… identified while constructing the consensus, the nucleotide call … assembly speed and quality,
requiring the shortest assembly … amount of data to assemble but still preserved enough for …
requiring the shortest assembly … amount of data to assemble but still preserved enough for …
A bioinformatics pipeline for sequence-based analyses of fungal biodiversity
DL Taylor, S Houston - Fungal Genomics: Methods and Protocols, 2011 - Springer
… Select all the contigs created at the assembly step, then use File → Export → Consensus
Sequences as Single File command, making sure to check the box for “export quality scores” (…
Sequences as Single File command, making sure to check the box for “export quality scores” (…
[HTML][HTML] Combining Transcriptome Assemblies from Multiple De Novo Assemblers in the Allo-Tetraploid Plant Nicotiana benthamiana
… The general consensus from these studies is that in order to reconstruct a broad range of …
the EvidentialGene tr2aacds pipeline maximises the diversity of de novo assembled transcripts …
the EvidentialGene tr2aacds pipeline maximises the diversity of de novo assembled transcripts …