A complete bacterial genome assembled de novo using only nanopore sequencing data

NJ Loman, J Quick, JT Simpson - Nature methods, 2015 - nature.com
… for de novo assembly owing to the lack of bioinformatics tools … Our assembly pipeline runs
this correction process multiple … Our consensus procedure starts with the initial draft assembly

Trycycler: consensus long-read assemblies for bacterial genomes

RR Wick, LM Judd, LT Cerdeira, J Hawkey, G Méric… - Genome biology, 2021 - Springer
… a consensus assembly from multiple input assemblies of the … Trycycler assemblies contained
fewer errors than assemblies … As assembly is often the first step in bioinformatic pipelines, …

Bioinformatics challenges of new sequencing technology

M Pop, SL Salzberg - Trends in genetics, 2008 - cell.com
… the standard overlap-layout-consensus approach to genome assembly, a paradigm that treats
… ] using a data analysis pipeline initially developed for Sanger data. Specifically, the ESTs …

Apollo: a sequencing-technology-independent, scalable and accurate assembly polishing algorithm

C Firtina, JS Kim, M Alser, D Senol Cali, AE Cicek… - …, 2020 - academic.oup.com
… Finding the path with the highest probability reveals the consensus of the aligned reads to
… a Canu-generated assembly is the best pipeline (ie one can construct the most accurate …

Reference-guided de novo assembly approach improves genome reconstruction for related species

HEL Lischer, KK Shimizu - BMC bioinformatics, 2017 - Springer
… genome and then used to construct an alternative consensus sequence [21]. This approach
… can be achieved with our reference-guided de novo assembly pipeline using ALLPATHS-LG…

V-pipe: a computational pipeline for assessing viral genetic diversity from high-throughput data

S Posada-Céspedes, D Seifert, I Topolsky… - …, 2021 - academic.oup.com
… On the other hand, limitations of de novo assembly include increased … consensus sequences
in case an alternative aligner is chosen. We use lowercase characters in the consensus

Consensus assessment of the contamination level of publicly available cyanobacterial genomes

L Cornet, L Meunier, M Van Vlierberghe, RR Léonard… - PLoS one, 2018 - journals.plos.org
pipeline combining six methods in a consensus strategy to assess the contamination level of
440 genome assemblies … , [14]), bioinformatic procedures are still needed to recognize and …

HINGE: long-read assembly achieves optimal repeat resolution

GM Kamath, I Shomorony, F Xia, TA Courtade… - Genome …, 2017 - genome.cshlp.org
… our assembly with the assembly produced by the NCTC pipeline (HGAP followed by Circlator)
on 10 randomly selected data sets. We verify that the assemblies … to generate consensus

NeatFreq: reference-free data reduction and coverage normalization for De Novosequence assembly

JM McCorrison, P Venepally, I Singh, DE Fouts… - … bioinformatics, 2014 - Springer
… were included in the assembled consensus while also reducing the … pipeline enables the
selection of the best available high confidence (quality) consensus sequences from assemblies

From reads to operational taxonomic units: an ensemble processing pipeline for MiSeq amplicon sequencing data

M Mysara, M Njima, N Leys, J Raes, P Monsieurs - Gigascience, 2017 - academic.oup.com
… challenges have been combined into various bioinformatics pipelines, such as mothur, …
paired-end assembly, by merging both forward and reverse reads into one consensus sequence, …