… CRISPR/Cas9 system can advance cotton functional genomic research and substantially
increase the potential for cotton … detected in cotton protoplasts could also be detected in plants, …

… understanding of Cas9 specificity is very limited in Cas9-edited plants. … Cas9-edited cotton
plants targeted to three genes, and three negative (Ne) control and three wild-type (WT) plants…

… achieved in cotton with … CRISPR/Cas9 system to produce targeted gene fragment deletions
in the GhPDS locus. Finally, we obtained transgenic cotton plants containing CRISPR/Cas9-…

… as pBeYDV-Cas9-KO and in the second vector, the ubiquitin promoter that drives Cas9 protein
was … The results from transgenic cotton calli/plants revealed that pBeYDV-Cas9-KO vector …

… In this study, we successfully utilized the CRISPR/Cas9 system in allotetraploid cotton and
accomplished multiple sites genome editing. The gene DsRed2 was chosen as a target. Firstly…

… in order to reduce workload and facilitate genome editing approaches in cotton. … in
CRISPR/Cas9-mediated target mutagenesis. Furthermore, we optimized the cotton CRISPR/Cas9 …

… of major challenges in cotton for repressing gene … cotton breeding scientists. The
clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) …

… commercial cotton … CRISPR/Cas9 system to modify the cotton genome. We made use of
the Green Fluorescent Protein (GFP) gene as the target that is already integrated into the cotton …

… cotton genome using CRISPR/Cas9 system. This chapter will employ GhMYB25-like A and
GhMYB25-like D that derived from upland cotton (… Put those transformed cotton plants in the …

… Overall, the results of this research lay the foundation for using the multiplexing approach
for the CRISPR based editing of cotton plants. In Pakistan, the CRISPR/Cas9 technology is still …