… We found that the Type IE CRISPR-Cas system in E. coli can be repurposed for programmable
gene repression through the deletion of cas3 and constitutive expression of the Cascade …

… More generally, we establish that transcriptional repression is inducible, reversible, and can
target essential genes. We demonstrate that we can use CRISPRi and CRISPRa to control …

… of gene function and to the construction of synthetic gene networks with desired properties.
Cas9 is an RNA-guided double-stranded DNA nuclease that participates in the CRISPR-Cas …

… Here, we developed the CRISPR interference system for gene repression in … gene repression
in Pseudomonas using S. pasteurianus dCas9. We demonstrate robust gene repression of …

… function of essential genes in archaea… CRISPR-Cas system IB can be harnessed to repress
gene expression in archaea. Deletion of cas3 and cas6b genes results in efficient repression …

… years, and gene repression systems based on the endogenous type I CRISPR/Cas system
have … In this study, a typical type IE CRISPR/Cas system was identified by analyzing the G. …

… genes. Although we observed varying degrees of gene repression depending on the target
gene, the dCas9 repressor consisting of KRAB and the transcription-repression domain of …

… for gene repression in Synechocystis. We show that genes can be effectively repressed in
… we found that a tightly repressed, low-strength promoter was most suitable for reversible …

… its endogenous type IB CRISPR-Cas system. Here, we present the two possible approaches
for gene repression as well as our workflow to achieve and assess gene knockdown, offer …

… (b) Trans-activating CRISPR RNA (tracrRNA):scaRNA-mediated gene repression. The 5′
end of the tracrRNA is homologous to the repeat sequences (yellow line). This allows its …