… We examine how the history of RNAi can inform today's challenges in CRISPR-Cas9 genome
engineering such as efficiency, specificity, high-throughput screening and delivery for in …

… They document development of the CRISPR-Cas system into a facile genome engineering
… genome engineering in animals and plants using RNA-programmable CRISPR-Cas9. The …

… (CRISPR) adaptive immune system can be used to facilitate efficient genome engineering in
… Here we describe a set of tools for Cas9-mediated genome editing via nonhomologous end …

… in genome engineering technologies based on the CRISPR-associated RNA-guided
endonuclease Cas9 are enabling the systematic interrogation of mammalian genome function. …

… genomic sequences. In this chapter, detailed protocols are described for harnessing …
CRISPR-Cas9 system from Streptococcus pyogenes to enable RNA-guided genome engineering …

… genome engineering using the clustered, regularly interspaced, short palindromic repeats
(CRISPR)-… in a variety of genomes 1,2 . Key modulators of the system include the choice of …

… Genome engineering through CRISPR/Cas9 technology in the human germline and … [The
application of CRISPR/Cas9 genome editing technology in cancer research]. Yi chuan …

… Emerging genome engineering tools have also advanced our ability to visualize genomic
sequences. For example, by fusing fluorescent proteins to TALEs, several groups have been …

… CRISPR–Cas9 has been broadly showed as a facile method for marker-free … genome
engineering of many organisms. Here, we have presented our method for CRISPR–Cas9 genome …

… medical researchers to edit parts of the genome by removing, adding, or … CRISPR-Cas9
in the field of medical research. Compared with other gene-editing technologies, CRISPR/Cas9 …