The new frontier of genome engineering with CRISPR-Cas9
JA Doudna, E Charpentier - Science, 2014 - science.org
… The advent of genomic sequencing technologies and the rapid generation of whole-genome
sequencing data for large numbers and types of organisms, including humans, has been …
sequencing data for large numbers and types of organisms, including humans, has been …
Outbred genome sequencing and CRISPR/Cas9 gene editing in butterflies
… Integrating comparative genomics and analyses of gene expression yields multiple insights
into butterfly evolution. We develop an efficient and widely applicable CRISPR/Cas9 gene …
into butterfly evolution. We develop an efficient and widely applicable CRISPR/Cas9 gene …
[HTML][HTML] Whole-genome sequencing analysis reveals high specificity of CRISPR/Cas9 and TALEN-based genome editing in human iPSCs
C Smith, A Gore, W Yan, L Abalde-Atristain, Z Li, C He… - Cell stem cell, 2014 - cell.com
… activities by Cas9 or TALENs. Our results from whole-genome sequencing analysis of Cas9-
and … that these engineered endonucleases provide efficient genome-editing tools with high …
and … that these engineered endonucleases provide efficient genome-editing tools with high …
Genome sequencing and CRISPR/Cas9 gene editing of an early flowering Mini‐Citrus (Fortunella hindsii)
… We report the first de novo assembled 373.6 Mb genome sequences (Contig-N50 2.2 Mb
and … Furthermore, the CRISPR/Cas9 system was demonstrated to be an efficient strategy to …
and … Furthermore, the CRISPR/Cas9 system was demonstrated to be an efficient strategy to …
CRISPR/Cas9 for genome editing: progress, implications and challenges
F Zhang, Y Wen, X Guo - Human molecular genetics, 2014 - academic.oup.com
… TALENs, CRISPR/Cas9 depends on small RNA for sequence-… to generate sequence
specificity, CRISPR/Cas9 is easily … challenges of CRISPR/Cas9-mediated genome editing and …
specificity, CRISPR/Cas9 is easily … challenges of CRISPR/Cas9-mediated genome editing and …
[HTML][HTML] Development and applications of CRISPR-Cas9 for genome engineering
… assembled to target specific sequences. ZF and … Cas9 nuclease from the microbial CRISPR
adaptive immune system is localized to specific DNA sequences via the guide sequence …
adaptive immune system is localized to specific DNA sequences via the guide sequence …
Identifying a large number of high-yield genes in rice by pedigree analysis, whole-genome sequencing, and CRISPR-Cas9 gene knockout
… Here we developed a method combining pedigree analysis, whole-genome sequencing,
and CRISPR-Cas9 technology. By sequencing the parents and descendants of IR8, the Green …
and CRISPR-Cas9 technology. By sequencing the parents and descendants of IR8, the Green …
Low incidence of off-target mutations in individual CRISPR-Cas9 and TALEN targeted human stem cell clones detected by whole-genome sequencing
… CRISPR-Cas9 and TALEN targeted human pluripotent stem cell clones, we performed
whole-genome sequencing … the degree of mutagenesis across the entire genome. In both types of …
whole-genome sequencing … the degree of mutagenesis across the entire genome. In both types of …
Recent progress in CRISPR/Cas9 technology
Y Mei, Y Wang, H Chen, ZS Sun, XD Ju - … of Genetics and Genomics, 2016 - Elsevier
… the genome sequence by using sgRNAs as the genomic anchor probe; 2) a light-sensitive
cryptochrome 2 (CRY2), either full-length CRY2 (CRY2FL) or a truncated CRY2 containing a …
cryptochrome 2 (CRY2), either full-length CRY2 (CRY2FL) or a truncated CRY2 containing a …