… coli, determine the specificities of 24 diverse anti-CRISPR proteins… application of CRISPR
technologies across their many uses. … have been slow to be adopted as CRISPR technologies. …

… Traditional CRISPR experiments in vivo require laboratory experience and … free
transcription-translation (TXTL) can be used to rapidly prototype and characterize CRISPR …

… Gene editing technologies such as zinc finger nucleases (ZFNs) and transcription
activator-like effector nucleases (TALENs) were among the first site-specific nucleases to be …

… transcriptional modulation. In this review, we present the brief history and basic mechanisms
of the CRISPR/… RNA, (2) mRNA for Cas9 translation alongside a separate guide RNA, and (3…

… constructs leads to active transcription and translation of functional biomolecules within minutes
… of CRISPR-Cas immune systems and advance the capabilities of CRISPR technologies. …

… coli cell-free transcription-translation system (TXTL) to rapidly elucidate … CRISPR nucleases,
with the potential to greatly accelerate our ability to characterize and harness novel CRISPR …

… , transcription activator-like effector nucleases and CRISPR–Cas … In the past decade, the
CRISPR–Cas9 technology has … because the target specificity of CRISPR–Cas9 relies on base …

… (CRISPR interference, CRISPRi) or fused with transcription … recent advances of these
CRISPR technologies, their delivery … Finally, the challenges to translate CRISPR to regenerative …

… Here, we hypothesized that simultaneous inhibition of transcription and translation can … for
transcription abortion and (ii) the L7Ae:K-turn system to self-repress the cas9 mRNA translation…

… -based trancriptional factors can control gene transcription … vector or CRISPR-mediated
transcription activation can … a CRISPR-dCasRx-based method to target protein translation of …