The design and synthesis of circular RNAs

P Obi, YG Chen - Methods, 2021 - Elsevier
Circular RNAs (circRNAs) are a novel class of RNAs distinguished … synthesized circRNAs
for specific biological roles. We focus on the most common applications, including designing

Optimal design of synthetic circular RNAs

SW Choi, JW Nam - Experimental & Molecular Medicine, 2024 - nature.com
circular RNAs and summarizes the available options for each step of exogenous circular RNA
design and synthesis… this review describes several potential applications of circular RNAs. …

Circular RNA: An emerging frontier in RNA therapeutic targets, RNA therapeutics, and mRNA vaccines

X Liu, Y Zhang, S Zhou, L Dain, L Mei, G Zhu - Journal of Controlled …, 2022 - Elsevier
Circular RNAs (circRNA) is a class of natural (biogenic) or synthetic closed RNA without 5′ …
(Adapted from Litke, JL and SR Jaffrey, Highly efficient expression of circular RNA aptamers …

Design and Synthesis of Circular RNA Expression Vectors

J Frei, NT Jarzebska, M Mellett, TM Kündig… - RNA Vaccines: Methods …, 2024 - Springer
… The increased interest in mRNA-based technologies has spurred a renaissance for circular
RNA (circRNA), as the lack of free 5′ and 3′ ends substantially increases resistance …

Characterizing and circumventing sequence restrictions for synthesis of circular RNA in vitro

JW Rausch, WF Heinz, MJ Payea… - Nucleic Acids …, 2021 - academic.oup.com
… in T4 td PIE construct design. Based on the results of our analysis, we present a set of
guidelines for designing PIE transcription templates for efficient synthesis of almost any circRNA, …

Circular RNAs as therapeutic agents and targets

LM Holdt, A Kohlmaier, D Teupser - Frontiers in physiology, 2018 - frontiersin.org
… It has recently been reported that thousands of covalently linked circular RNAs (circRNAs)
are … which designer functions could be engineered into artificial therapeutic circular RNAs. …

Synthesis and engineering of circular RNAs

S Petkovic, S Müller - Circular RNAs: Methods and Protocols, 2018 - Springer
… Here we describe the chemical synthesis of a small RNA (49 nts) site-specifically modified
with 5-fluorouridine followed by circularization with T4 RNA ligase I (Fig. 2A). Furthermore, we …

Engineering circular RNA for enhanced protein production

R Chen, SK Wang, JA Belk, L Amaya, Z Li… - Nature …, 2023 - nature.com
Circular RNAs (circRNAs) are stable and prevalent RNAs in eukaryotic cells that arise from
back-splicing. Synthetic circRNAs and some endogenous circRNAs can encode proteins, …

Chemical Synthesis of Circular RNAs with Phosphoramidate Linkages for Rolling‐Circle Translation

K Nakamoto, H Abe - Current Protocols, 2021 - Wiley Online Library
… of the circular RNAssynthesis and purification of circular RNAs for rolling-circle translation
(Nakamoto et al., 2020). This approach enables a more practical synthesis of circular RNA

Circular RNA: Design Criteria for Optimal Therapeutical Utility

EM Carmona - 2019 - search.proquest.com
Circular RNA extends protein expression longer than linear modified RNA in vivo ........... 69
… Since circular RNA is covalently linked at its 5’-3’ end it is resistance to RNase R digestion. (D…