Differential purification of autocrine motility factor derived from a murine protein-free fibrosarcoma

H Watanabe, K Kanbe, M Chigira - Clinical & Experimental Metastasis, 1994 - Springer
… (s) of motility molecule that stimulates the cells in an autocrine fashion remains unknown. In
the present paper, we report the purification to homogeneity of two motility factors, which act …

Purification of Motility Factor (GMF) from Human Malignant Glioma-Cells and Its Biological Significance in Tumor Invasion

T Ohnishi, N Arita, T Hayakawa, K Kawahara… - Biochemical and …, 1993 - Elsevier
… Following the incubation, the filters were removed and the cells were fixed and stained with
Diff… After incubation for 20 hours at 37C in 5% CO2, the filters were fixed and stained with Diff-…

Alterations in Vibrio cholerae motility phenotypes correlate with changes in virulence factor expression

CL Gardel, JJ Mekalanos - Infection and immunity, 1996 - Am Soc Microbiol
… and purified to single colonies on LB agar plates and then retested on LB motility agar plates
… , and plated on appropriate selective or differential media to allow separate enumeration of …

A sensitive screening assay for secreted motility‐stimulating factors

SW Nam, T Clair, E Schiffmann… - Cell Motility and the …, 2000 - Wiley Online Library
… A number of such autocrine motility factors have now been … factors have been purified and
cloned: the insulin-like growth … , fixed, and stained with Diff-Quik reagents, then mounted on …

Protein factors which regulate cell motility

EM Rosen, ID Goldberg - In vitro cellular & developmental biology, 1989 - Springer
… the multiplicity of different protein factors which directly or indirectly affect motility of mammalian
cells. The availability of purified cell motility factors will facilitate understanding of the …

Purification and biological characterization of epitaxin, a fibroblast-derived motility factor for epithelial cells.

M Shimonaka, Y Yamaguchi - Journal of Biological Chemistry, 1994 - Elsevier
… After an 18-h incubation, the cells were fixed, stained with Diff-Quick, and analyzed for …
described the purification and biological characterization of epitaxin, a 36-kDa motility factor for …

Expression and secretion of neuroleukin/phosphohexose isomerase/maturation factor as autocrine motility factor by tumor cells

Y Niinaka, S Paku, A Haga, H Watanabe, A Raz - Cancer research, 1998 - AACR
Purification of B16-F1 melanoma uutocrine motility factor and … Purification of human
tumor cell autocrine motility factor and … Autocrine motility factor induces differential 12-lipoxygenasc …

Tumor cell autocrine motility factor is the neuroleukin/phosphohexose isomerase polypeptide

H Watanabe, K Takehana, M Date, T Shinozaki, A Raz - Cancer research, 1996 - AACR
… Here, we report on the purification and partial peptide sequencing of a murine fibro- …
stimulation activity with that of purified AMF. Fig. 2 demonstrates that the motile-inducing activ ities of …

… rpf Clusters Distributed among a Population of Stenotrophomonas maltophilia Clinical Strains Display Differential Diffusible Signal Factor Production and Virulence …

P Huedo, D Yero, S Martínez-Servat… - Journal of …, 2014 - Am Soc Microbiol
… mutant shows reduced swimming motility, reduced exoprotease … motility phenotype of
Burkholderia cenocepacia (9). … extraction with a GeneJet RNA purification kit (Thermo Scientific), …

Differential expression of virulence genes and motility in Ralstonia (Pseudomonas) solanacearum during exponential growth

SJ Clough, AB Flavier, MA Schell… - Applied and …, 1997 - Am Soc Microbiol
… We report here that two PhcA-regulated virulence factors, endoglucanase (Egl) and acidic
exopolysaccharide I (EPS I), and motility are expressed differentially during exponential …