DNA methylation in plants: mechanisms and tools for targeted manipulation

J Gallego‐Bartolomé - New Phytologist, 2020 - Wiley Online Library
… loci through programmable DNA-binding proteins have expanded the tool kit … DNA methylation
in plants and discusses the tools that have recently been developed for its manipulation. …

[HTML][HTML] Epigenome editing: targeted manipulation of epigenetic modifications in plants

H Shin, WL Choi, JY Lim, JH Huh - Genes & Genomics, 2022 - Springer
… Epigenome editing strategy for targeted manipulation of DNA methylation and histone …
aimed at editing the epigenome in plants and discuss the potentials of epigenetic manipulation for …

CRISPR-dCas9-based targeted manipulation of DNA methylation in plants

B Ghoshal, J Gardiner - CRISPR-Cas Methods: Volume 2, 2021 - Springer
… protocol for targeted manipulation of DNA methylation by … lab for the targeted manipulation
of DNA methylation in Arabidopsis… further study into the function of DNA methylation in plants. …

[HTML][HTML] Induced methylation in plants as a crop improvement tool: progress and perspectives

C Mercé, PE Bayer, C Tay Fernandez, J Batley… - Agronomy, 2020 - mdpi.com
… variation such as DNA methylation on plant phenotype has … However, their manipulation is
still costly and labor intensive, … Inducing targeted DNA methylation to modify the expression …

[HTML][HTML] Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems

A Papikian, W Liu, J Gallego-Bartolomé… - Nature …, 2019 - nature.com
plants. Here, we adapt the dCas9-SunTag system to engineer targeted gene activation and
DNA methylation … present a CRISPR-based methylation targeting system for plants, utilizing a …

[HTML][HTML] DNA methylation can alter CRISPR/Cas9 editing frequency and DNA repair outcome in a target‐specific manner

A Přibylová, L Fischer, DE Pyott, A Bassett… - The New …, 2022 - ncbi.nlm.nih.gov
… Our findings suggest that DNA methylation can indirectly impair Cas9 activity and subsequent
DNA repair… RNA virus to induce DNA methylation by triggering the plant’s RNA‐dependent …

[HTML][HTML] Ectopic targeting of CG DNA methylation in Arabidopsis with the bacterial SssI methyltransferase

W Liu, J Gallego-Bartolomé, Y Zhou, Z Zhong… - Nature …, 2021 - nature.com
… The ability to target epigenetic marks like DNA methylation to specific loci is important in …
and in crop plant engineering. However, heritability of targeted DNA methylation, how it impacts …

CRISPR-based targeting of DNA methylation in Arabidopsis thaliana by a bacterial CG-specific DNA methyltransferase

B Ghoshal, CL Picard, B Vong… - Proceedings of the …, 2021 - National Acad Sciences
… both in crop engineering and in plant genetic research. … To test the hypothesis that targeting
methylation directly to … -based CG-specific targeted DNA methylation system for plants. A CG-…

[HTML][HTML] Chromatin manipulation and editing: challenges, new technologies and their use in plants

K Fal, D Tomkova, G Vachon, ME Chaboute… - International Journal of …, 2021 - mdpi.com
methylation windows [141]. Interestingly, while a consistent off-target DNA methylation was
… exhibits much higher specificity and DNA methylation induction at the target sites [142]. Also…

[HTML][HTML] RNA-directed DNA methylation

RM Erdmann, CL Picard - PLoS genetics, 2020 - journals.plos.org
DNA methylation in plants is typically divided into three … targeted by several DNA methylation
pathways in plants. … or manipulation, suggesting that RdDM can be a valuable tool for …