Over-production of stereoselective nitrile hydratase from Pseudomonas putida 5B in Escherichia coli : activity requires a novel downstream protein

S Wu, RD Fallon, MS Payne - Applied microbiology and biotechnology, 1997 - Springer
Downstream region required for NHase activity To determine how much of the downstream
sequence … varying lengths of downstream sequence (Fig. 2). Constructs with at least 0.5 kb of …

… of combination of different− 10 hexamers and downstream sequences on stationary-phase-specific sigma factor ςS-dependent transcription in Pseudomonas putida

EL Ojangu, A Tover, R Teras, M Kivisaar - Journal of Bacteriology, 2000 - Am Soc Microbiol
… Therefore, taking together the published data and the results presented in this study, we
propose that the possible role of downstream sequences in ς S -dependent transcription needs …

Molecular characterization of Pseudomonas putida KT2440 rpoH gene regulation

M Manzanera, I Aranda-Olmedo, JL Ramos… - …, 2001 - microbiologyresearch.org
… We inserted a 50 bp linker, with the inverted repeat and the T-track sequence located
downstream of rpoH, between the λp′R promoter and the lacZ reporter gene, to produce pMPRH. …

[HTML][HTML] Engineering the Osmotic State of Pseudomonas putida KT2440 for Efficient Cell Disruption and Downstream Processing of Poly(3-Hydroxyalkanoates)

I Poblete-Castro, C Aravena-Carrasco… - … in Bioengineering and …, 2020 - frontiersin.org
… costly enzymes and mechanical disrupters for downstream processing of intracellular microbial
… Herein the osmotic state of Pseudomonas putida KT2440 was engineered by inactivating …

[HTML][HTML] Industrial biotechnology of Pseudomonas putida: advances and prospects

A Weimer, M Kohlstedt, DC Volke, PI Nikel… - Applied Microbiology …, 2020 - Springer
Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered
in … throughout central carbon metabolism of glucose-grown Pseudomonas putida. The P. …

[HTML][HTML] Development of a high efficiency integration system and promoter library for rapid modification of Pseudomonas putida KT2440

JR Elmore, A Furches, GN Wolff, K Gorday… - Metabolic engineering …, 2017 - Elsevier
… caused by this DNA recombinase raised concerns about its downstream usefulness. As the
… pPolyAttP plasmid integration efficiency, we used this strain for all downstream experiments. …

Transcriptional regulation, nucleotide sequence, and localization of the promoter of the catBC operon in Pseudomonas putida

TL Aldrich, AM Chakrabarty - Journal of bacteriology, 1988 - Am Soc Microbiol
… the catB structural gene, 214 bp downstream of the ATG initiation codon (1). DNA sequencing
was begun from the XhoI site and continued downstream for 2,040 bp through a Sall site. …

A Set of Activators and Repressors Control Peripheral Glucose Pathways in Pseudomonas putida To Yield a Common Central Intermediate

T Del Castillo, E Duque, JL Ramos - Journal of bacteriology, 2008 - Am Soc Microbiol
Pseudomonas putida KT2440 channels glucose to the central Entner-Doudoroff intermediate
6-phosphogluconate through three convergent pathways. The genes for these convergent …

Analysis of Pseudomonas putida alkane-degradation gene clusters and flanking insertion sequences: evolution and regulation of the alk genes

JB van Beilen, S Panke, S Lucchini… - …, 2001 - microbiologyresearch.org
… In P. putida P1, the alkBFGHJKL and alkST gene clusters are flanked by almost identical
copies of the insertion sequence ISPpu4, constituting a class 1 transposon. Other insertion …

[HTML][HTML] Pseudomonas putida—a versatile host for the production of natural products

A Loeschcke, S Thies - Applied microbiology and biotechnology, 2015 - Springer
Pseudomonas putida has emerged as a microbial laboratory work horse, with elaborated …
This review provides an overview of applications of P. putida as a host organism for the …