Illuminating the sites of enterovirus replication in living cells by using a split-GFP-tagged viral protein

HM Van der Schaar, CE Melia, JAC Van Bruggen… - MSphere, 2016 - Am Soc Microbiol
… To overcome this limitation, we used a split green fluorescent protein (split-GFP) … ; GFP(S1-10)]
and a small fragment [strand 11; GFP(S11)] of only 16 residues. The GFP(S11) (GFP with …

[PDF][PDF] ILLUMINATING THE SITES OF ENTEROVIRUS REPLICATION IN LIVING CELLS BY USING A SPLIT-GFP-TAGGED VIRAL PROTEIN

US INFEC - scholarlypublications …
… In conclusion, we have presented split-GFP tagged CVB3 as a new tool for the direct
visualization of enterovirus ROs in living cells, which will allow the study of their dynamics. …

Origins of enterovirus replication organelles established by whole-cell electron microscopy

CE Melia, CJ Peddie, AWM de Jong, EJ Snijder… - MBio, 2019 - Am Soc Microbiol
… and genome replication. Our data provide direct evidence that enteroviruses use ER and
then … Illuminating the sites of enterovirus replication in living cells by using a split-GFP-tagged …

Enterovirus 71‐induced autophagy detected in vitro and in vivo promotes viral replication

SC Huang, CL Chang, PS Wang… - Journal of medical …, 2009 - Wiley Online Library
Enterovirus 71 (EV71) is an important pathogen causing … punctate GFP-microtubule-associated
protein 1 light chain 3 (GFP-… Autophagy induced by EV71 is beneficial for viral replication. …

Host factors in enterovirus 71 replication

SR Shih, V Stollar, ML Li - Journal of virology, 2011 - Am Soc Microbiol
Enterovirus 71 (EV71) infections continue to remain an … This has spurred much fundamental
research into the replication of … Host cell proteins play an important role in the replication of …

Enterovirus infection induces massive recruitment of all isoforms of small cellular arf GTPases to the replication organelles

S Moghimi, E Viktorova, A Zimina, T Szul… - Journal of …, 2020 - Am Soc Microbiol
… It should be noted that sometimes we observed additional weak GFP-positive bands on the
… process, was localized adjacent to the Arf-enriched areas but was often strictly separated in …

[PDF][PDF] THE EMERGENCE OF ENTEROVIRUS REPLICATION ORGANELLES CAPTURED BY WHOLE-CELL ELECTRON MICROSCOPY

GOF ENTER, US REPLIC, UBYWC MICR - scholarlypublications …
… proliferation and genome replication. Our data provide direct evidence that enteroviruses use
ER … to monitor the emergence of viral 3A protein using a split-GFP system. The resulting 3A-…

A second open reading frame in human enterovirus determines viral replication in intestinal epithelial cells

H Guo, Y Li, G Liu, Y Jiang, S Shen, R Bi… - Nature …, 2019 - nature.com
… 9); ectopically expressed ORF2p co-localized with LC3-GFP and was sufficient for autophagy
… The autophagy pathway is known to be important for enterovirus replication at different …

TRIM7 inhibits enterovirus replication and promotes emergence of a viral variant with increased pathogenicity

W Fan, KB Mar, L Sari, IK Gaszek, Q Cheng, BM Evers… - Cell, 2021 - cell.com
enteroviruses. We reveal the mechanism by which TRIM7 suppresses enterovirus replication
… We cloned T323A into the CVB3-GFP molecular clone and found that the resulting virus, …

Escaping host factor PI4KB inhibition: enterovirus genomic RNA replication in the absence of replication organelles

CE Melia, HM Van der Schaar, H Lyoo, RWAL Limpens… - Cell reports, 2017 - cell.com
… proposed role of ROs in shielding enteroviral RNA from cellular sensors. Neither … for enterovirus
genome replication and immune evasion. … For this, we employed the split-GFP system ( …