Rational design of enzyme activity and enantioselectivity
… applying rational design strategy has been increasing. Here, we reviewed the recent
advances of applying the rational design strategy to engineer enzyme functions including activity …
advances of applying the rational design strategy to engineer enzyme functions including activity …
Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
… Rational design, semi-rational design or whole-gene randomization each refer to multiple
methodologies, as outlined in the text. The enzyme … -rational design of enzyme activities has …
methodologies, as outlined in the text. The enzyme … -rational design of enzyme activities has …
Rational design of practically important enzymes
VI Tishkov, AA Pometun, AV Stepashkina… - Moscow University …, 2018 - Springer
… of interest and to improve the enzyme properties for a particular … based on bioinformatics
and rational design. New genes … sp.101 enzyme (PseFDH) yields enzyme preparations with …
and rational design. New genes … sp.101 enzyme (PseFDH) yields enzyme preparations with …
Rational design strategies for nanozymes
Z Chen, Y Yu, Y Gao, Z Zhu - ACS nano, 2023 - ACS Publications
… –activity relationship of natural enzymes to the design of … activities and rational prediction
of the nanozyme performance. Biomimetic design has unique advantages for the design …
of the nanozyme performance. Biomimetic design has unique advantages for the design …
Toward rational design of high-efficiency enzyme cascades
… enzymatic activity. A necessary control is to determine the activity of a mixture in which the
enzymes … an increased final activity indicates that the activity of at least one enzyme must have …
enzymes … an increased final activity indicates that the activity of at least one enzyme must have …
Enzyme engineering for enantioselectivity: from trial-and-error to rational design?
LG Otten, F Hollmann, IWCE Arends - Trends in biotechnology, 2010 - cell.com
… De novo design The ultimate goal of enzyme engineering is a true rational design, which
aims at de novo engineering of (enantioselective) enzymes. The scope of this approach is …
aims at de novo engineering of (enantioselective) enzymes. The scope of this approach is …
Rational-design engineering to improve enzyme thermostability
V Pongsupasa, P Anuwan, S Maenpuen… - Enzyme Engineering …, 2022 - Springer
… demonstrate a rational-design strategy by site-directed mutagenesis that mutates specific
residues using a pair of complementary primers to generate mutants, in which primer design is …
residues using a pair of complementary primers to generate mutants, in which primer design is …
Structure-guided rational design of the substrate specificity and catalytic activity of an enzyme
JM Choi, HS Kim - Methods in Enzymology, 2020 - Elsevier
… success rate in generating an enzyme with desired property. In … in rational design of an
enzyme, and present some applications to the design of substrate specificity and catalytic activity …
enzyme, and present some applications to the design of substrate specificity and catalytic activity …
Multienzyme‐like nanozymes: regulation, rational design, and application
J Sheng, Y Wu, H Ding, K Feng, Y Shen… - Advanced …, 2024 - Wiley Online Library
… to develop and design nanozymes with multiple-enzyme activities and their … activity regulation,
rational design, and recent application progress of nanozymes with multienzyme activities…
rational design, and recent application progress of nanozymes with multienzyme activities…
Rational Design of Enzyme‐Modified Electrodes for Optimized Bioelectrocatalytic Activity
… of cys-BOD makes these rationally designed enzyme-modified electrodes very promising
candidates for high-performance bioelectrocatalytic devices with improved activity and stability. …
candidates for high-performance bioelectrocatalytic devices with improved activity and stability. …
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