… applying rational design strategy has been increasing. Here, we reviewed the recent
advances of applying the rational design strategy to engineer enzyme functions including activity …

… Rational design, semi-rational design or whole-gene randomization each refer to multiple
methodologies, as outlined in the text. The enzyme … -rational design of enzyme activities has …

… of interest and to improve the enzyme properties for a particular … based on bioinformatics
and rational design. New genes … sp.101 enzyme (PseFDH) yields enzyme preparations with …

… –activity relationship of natural enzymes to the design of … activities and rational prediction
of the nanozyme performance. Biomimetic design has unique advantages for the design …

… enzymatic activity. A necessary control is to determine the activity of a mixture in which the
enzymes … an increased final activity indicates that the activity of at least one enzyme must have …

… De novo design The ultimate goal of enzyme engineering is a true rational design, which
aims at de novo engineering of (enantioselective) enzymes. The scope of this approach is …

… demonstrate a rational-design strategy by site-directed mutagenesis that mutates specific
residues using a pair of complementary primers to generate mutants, in which primer design is …

… success rate in generating an enzyme with desired property. In … in rational design of an
enzyme, and present some applications to the design of substrate specificity and catalytic activity …

… to develop and design nanozymes with multiple-enzyme activities and their … activity regulation,
rational design, and recent application progress of nanozymes with multienzyme activities…

… of cys-BOD makes these rationally designed enzyme-modified electrodes very promising
candidates for high-performance bioelectrocatalytic devices with improved activity and stability. …