… developing novel enzymatic activities. Catalytic efficiencies of man-made enzymes however
… The discrepancy between laboratory and naturally evolved enzymes suggests that a major …

… enzyme specificity. The response must be more guarded, though, in terms of redesigning
enzyme … recent trends in the rational redesign of enzyme substrate specificity and mechanisms, …

… In the following sections, I will focus mainly on what we know about enzymes, on how
they work, and on what rational enzyme engineering studies have told us about enzymes. …

… Here, we explore efforts to rationally modify enzymes … of enzymes where links between
enzyme function and structure have been made, thus enabling rational changes to the enzyme …

… enzyme engineering. A major challenge in engineering α-ketoglutarate-dependent oxygenases
is to develop a rational … , we report a strategy for rational redesign of a model enzyme, 4-…

… With the improvement of enzyme redesign algorithms, model-… advance the field of enzyme
redesign with the accumulation of … the field of computational enzyme redesign. We anticipate a …

… Rational design, semi-rational design or whole-gene randomization each refer to multiple
methodologies, as outlined in the text. The enzyme … semi-rational design of enzyme activities …

… rational and arational† redesign of these enzymes to create new biocatalysts of differing
specificities. Although these attempts have been useful in identifying structural elements that …

… first example in which rational redesign of residues, at or close to the active site, has been
used to demonstrate that the psychrophilic character of an enzyme can be strongly affected by …

… more ferrocenecarboxylic acid mediator to the enzyme resulting in improved stability and …
of the sequence and structure-function similarity in GOx and enzymes homologous to it from …