… Herein, we report on the asymmetric amination of various prochiral ketones by formal
reductive amination, employing ω-transaminases with known sequences. The ω-transaminases …

… The cloning vector pMD18-T (TaKaRa, Japan) was used for gene cloning and Escherichia
coli JM109 was used as host for this vector. The plasmid pET-28a with T7p/Olac promoter/…

… In this work, the asymmetric bioreduction of α-keto carboxylic acids to chiral α-hydroxy
carboxylic acids by using keto acid reductases was explored. A NADH-dependent α-keto acid …

… of strains of Escherichia coli requiring succinate for aerobic growth on glucose minimal
medium. One class, designated sucA, lacked the ~-ketoglutarate deearboxylase component (E 1) …

… Escherichia coli B was grown on stoCk slants containing micro inoculum broth (Difco) and
1·5 % agar and was kept in a freezer under glycerol between transfers. For the experiments …

… Here we report on production of such petroleum-replica hydrocarbons in Escherichia coli. …
of the branched-chain α-keto acid dehydrogenase complex and β-keto acyl-acyl carrier protein …

… -ketoglutarate dehydrogenation complexes of Escherichia coli and pointing to its association
… been obtained in a highly purified state from E. coli extracts and shown to be a flavoprotein. …

… Our results showed that YtbE accepts both α- and β-keto esters. In fact, a BLAST analysis
revealed that the primary structure of YtbE shows 47–34% identities to those reductases. …

… In this study, we cloned three genes encoding the three KADC variants into Escherichia coli
and characterized the corresponding recombinant protein. Additionally, homology models of …

… A soluble preparation capable of deamidating glutamine in the presence of a-keto acids has
… 14 A similar mechanism is involved in the a-keto acid-mediated deamidation of asparagine: …