Small-scale expression of proteins in E. coli
S Zerbs, S Giuliani, F Collart - Methods in enzymology, 2014 - Elsevier
… to succeed in large-scale purification cultures. Small-scale trials also aid in determining the
… as the same isolate can easily be assayed in multiple growth conditions with small cultures. …
… as the same isolate can easily be assayed in multiple growth conditions with small cultures. …
High level production of soluble single chain antibodies in small-scale Escherichia coli cultures
SM Kipriyanov, G Moldenhauer, M Little - Journal of immunological …, 1997 - Elsevier
We have investigated the effect of growth and induction conditions on the production of
soluble single-chain Fv antibody fragments in Escherichia coli under the control of wt lac …
soluble single-chain Fv antibody fragments in Escherichia coli under the control of wt lac …
High throughput construction and small scale expression screening of multi-tag vectors in Escherichia coli
LE Bird - Methods, 2011 - Elsevier
… These vectors in combination with a small scale Escherichia coli expression screen can be
used to assess in parallel the effect of fusion tags on solubility. The vectors were tested with …
used to assess in parallel the effect of fusion tags on solubility. The vectors were tested with …
A simple single-step procedure for small-scale preparation of Escherichia coli plasmids.
M He, A Wilde, MA Kaderbhai - Nucleic Acids Research, 1990 - ncbi.nlm.nih.gov
… of plasmid DNA on a miniscale, demanding no more than 20 minutes. The principle is based
on the finding that phenol/chloroform treatment of E. coli cells in the presence of LiCl and …
on the finding that phenol/chloroform treatment of E. coli cells in the presence of LiCl and …
High Prevalence of Antibiotic Resistance in Pathogenic Escherichia coli from Large- and Small-Scale Poultry Farms in Bangladesh
B Hasan, R Faruque, M Drobni… - Avian …, 2011 - meridian.allenpress.com
… of antibiotic resistance patterns in avian pathogenic Escherichia coli in Bangladesh. Of 279
dead or sick poultry of different ages, 101 pathogenic E. coli strains isolated from broilers and …
dead or sick poultry of different ages, 101 pathogenic E. coli strains isolated from broilers and …
Scale-up methodologies for Escherichia coli and yeast fermentation processes
BH Junker - Journal of bioscience and bioengineering, 2004 - Elsevier
… hygroscopicus when agitation rate was used to control DO as was done at the small scale
(19). SUDS syndrome included culture morphological changes which affected packed cell …
(19). SUDS syndrome included culture morphological changes which affected packed cell …
[HTML][HTML] Quantitative transfer of Escherichia coli O157: H7 to equipment during small-scale production of fresh-cut leafy greens
… large-scale farms in the Salinas Valley of California. In September 2006, contaminated
prebagged baby spinach triggered an Escherichia coli O157:… A few months later, two additional E. …
prebagged baby spinach triggered an Escherichia coli O157:… A few months later, two additional E. …
Scale‐up of Escherichia coli growth and recombinant protein expression conditions from microwell to laboratory and pilot scale based on matched kLa
RS Islam, D Tisi, MS Levy… - Biotechnology and …, 2008 - Wiley Online Library
… ABSTRACT: Fermentation optimization experiments are ideally performed at small scale to
… translate an optimized small-scale fermentation process to laboratory and pilot scale stirred-…
… translate an optimized small-scale fermentation process to laboratory and pilot scale stirred-…
[PDF][PDF] Scale-up of Escherichia coli fermentation from small scale to pilot scale using Eppendorf fermentation systems
B Li, M Sha - Eppendorf Application Note, 2016 - eppendorf.com
… Equipment The Eppendorf fermentation systems, from small scale to pilot scale, used in
this project are shown in Figure 1. Vessel parameters critical for scale-up are listed in Table 1. …
this project are shown in Figure 1. Vessel parameters critical for scale-up are listed in Table 1. …
The growing scope of applications of genome-scale metabolic reconstructions using Escherichia coli
AM Feist, BØ Palsson - Nature biotechnology, 2008 - nature.com
… In addition to analyses on the genomic scale, several studies modeling the metabolism of
E. coli on a smaller scale have been performed. These analyses typically use models …
E. coli on a smaller scale have been performed. These analyses typically use models …
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