Biofuel production in Escherichia coli: the role of metabolic engineering and synthetic biology

JM Clomburg, R Gonzalez - Applied microbiology and biotechnology, 2010 - Springer
Escherichia coli has become a promising host organism for the microbial production of …
metabolic engineering and synthetic biology have led to the ability to efficiently engineer E. …

ePathBrick: A Synthetic Biology Platform for Engineering Metabolic Pathways in E. coli

P Xu, A Vansiri, N Bhan, MAG Koffas - ACS synthetic biology, 2012 - ACS Publications
… in synthetic biology have greatly speeded up our ability to design and construct cell factories
for metabolic engineering application. To date, a number of synthetic biology approaches …

EcoFlex: A Multifunctional MoClo Kit for Ecoli Synthetic Biology

SJ Moore, HE Lai, RJR Kelwick, SM Chee… - … Synthetic Biology, 2016 - ACS Publications
coli. We have built a versatile Golden Gate toolbox based on the MoClo assembly
standard (9) for E. coli synthetic biology, which we make available through AddGene (www.addgene.…

Potential applications of the Escherichia coli heat shock response in synthetic biology

JL Rodrigues, LR Rodrigues - Trends in biotechnology, 2018 - cell.com
coli HSR mechanism in synthetic biology approaches. Several components of the regulatory
… on the potential of the E. coli HSR in conjunction with synthetic biology strategies in several …

Metabolic engineering and synthetic biology approaches driving isoprenoid production in Escherichia coli

C Wang, B Zada, G Wei, SW Kim - Bioresource Technology, 2017 - Elsevier
… Rapid developments in metabolic engineering and synthetic biology have facilitated the
engineering of isoprenoid biosynthetic pathways in Escherichia coli to induce high levels of …

Protocols for implementing an Escherichia coli based TX-TL cell-free expression system for synthetic biology

ZZ Sun, CA Hayes, J Shin, F Caschera… - JoVE (Journal of …, 2013 - jove.com
… for synthetic biology. To achieve the latter goal, cell-free expression systems that preserve
endogenous Escherichia coli … the preparation and execution of an efficient endogenous E. …

A synthetic biology approach to self-regulatory recombinant protein production in Escherichia coli

M Dragosits, D Nicklas, I Tagkopoulos - Journal of biological engineering, 2012 - Springer
… and cellular stress, we developed a synthetic expression platform that enables the cell to
shut … This study illustrates the potential of synthetic biology to help traditional biotechnological …

The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology

J Garamella, R Marshall, M Rustad… - … synthetic biology, 2016 - ACS Publications
… The goal of this work was first to construct the most versatile and powerful all E. coli cell-free
TX-TL systems for in vitro synthetic biology. We worked on four parts of the TX-TL reaction: …

Reduced evolvability of Escherichia coli MDS42, an IS-less cellular chassis for molecular and synthetic biology applications

K Umenhoffer, T Fehér, G Balikó, F Ayaydin… - Microbial cell …, 2010 - Springer
… run, it might be an undesirable feature in synthetic biological (SB) applications [1]. The SB
approach is ideally based on the notion that biological parts can be precisely designed and …

Metabolic engineering and synthetic biology for isoprenoid production in Escherichia coli and Saccharomyces cerevisiae

GR Navale, MS Dharne, SS Shinde - Applied Microbiology and …, 2021 - Springer
… Recent advancement in metabolic engineering and synthetic biology has enabled the
production of these isoprenoids in the heterologous host systems like Escherichia coli and …