… , in media containing either the antibiotic cefotaxime or piperacillin with clavulanic acid, the
optimum genotype of the antibiotic resistance protein TEM β-lactamase in Escherichia coli is …

… is a widely used research tool in systems and synthetic biology. However, if CFPS is to become
… based modeling to evaluate the performance of E. coli cell-free protein synthesis. A core …

… , for instance, and it is widely used in synthetic biology and metabolic engineering [6–9] to
… to Escherichia coli, the most prominent biotechnological host of interest, are discussed. E. …

… Here, we develop this strategy in Escherichia coli, with increased biosensor stability due
to ligand binding leading to increased expression of a metabolic reporter gene and hence …

… levels precisely hinders our ability to engineer biological systems. Using well-characterized …
on prediction or standardization, we can screen synthetic libraries for desired behavior. …

… (E) Systems biology, synthetic biology, and evolutionary engineering tools accelerate
metabolic flux optimization to maximize target chemical production. (F) Fermentation goes in …

… of Escherichia coli to express e-PNs by introducing a plasmid that contained an inducible
operon with E. … We thought that Escherichia coli might be an ideal chassis for e-PN fabrication. …

… Synthetic biology uses DNA programs to add new functions into living cells. By expressing
transcription factors, microbes such as Escherichia coli … In synthetic biology, the use of DNA …

… Synthetic biology and metabolic engineering have been extensively used in S. cerevisiae,
Zymomonas mobilis and Escherichia coli to enhance ethanol fermentation (Fig. 2) [27, 35–38]…

… in the fields of systems biology and synthetic biology, allowing the design of intricate synthetic
circuits and the interrogation of complex natural biological systems. Until recently, however…